中文摘要：

目的：探讨血管活性肠肽在三硝基苯磺酸诱导的肠易激综合征大鼠中的表达量及中药穿山龙提取物薯蓣皂苷的干预作用。方法：TNBS诱导大鼠肠易激综合征模型,第2、7、14和28天对大鼠体质量、髓过氧化物酶活力、疼痛阈值、VIP含量进行测定。结果：TNBS诱导的肠易激综合征大鼠体质量降低,髓过氧化物酶活力增加,疼痛阈值降低,模型大鼠中性粒细胞内炎症标记物过氧化物酶的表达比正常大鼠明显升高,证实TNBS处理后诱导了大鼠的炎症,TNBS处理大鼠后血浆VIP浓度比对照组显著增加（P〈0.05）,穿山龙提取物薯蓣皂苷处理后显著下调。结论：VIP表达的改变在肠易激综合症中可能起着重要作用,穿山龙提取物中药薯蓣皂苷对TNBS诱导的IBS大鼠具有治疗作用。

英文摘要：

Objective： To investigate the vasoactive intestinal peptides（ VIP） expression in irritable bowel syndrome（ IBS） rats induced by trinitrobenzene sulfonic acid（ TNBS）,and the intervention effects of dioscin from Rhizoma Dioscoreae Nipponicase. Methods： IBS models were established by TNBS induction. The weight,myeloperoxidase（ MPO）activity,pain threshold,and VIP content were detected on the days of 2nd,7th,14th,and 28th. Results： TNBS induced IBS in rats was confirmed with weight loss,increased MPO activity,and decreased pain threshold. VIP plasma concentration was significantly increased in rats following TNBS intervention compared to that of the control group（ P〈0. 05）,and it was regulated downwards significantly. Conclusion： VIP expression may play a role in IBS. Therefore,dioscin has therapeutic effect on IBS rats induced by TNBS.