中文摘要：

目的：对不同产地缬草中活性成分青剌尖木酯醇苷进行含量分析。方法：采用高效液相色谱法，Agilent HC-C18（4．6mm×250mm，5μm）柱；乙腈水（16：84）为流动相，流速为1．0mL·min^-1，检测波长229nm。结果：青刺尖木酯醇苷在0．304～1．520μg范围内线性关系良好（r=0．9999），平均回收率为99．89％，RSD为1．08％。结论：该方法简单、快速、灵敏、专属性强，能准确测定缬草中活性成分青剌尖木酯醇苷的含量。

英文摘要：

OBJECTIVE To develop a method for the determination of prJnsepiol-4-O-β-D-glucoside content in Valeriana officinalis L. METHODS HPLC was used. The chromatographic conditions were： Agilent HC-C18 Column （4. 6 × 250 mm, 5 μm）, acetonitrile - water （16：84） as mobile phase, flow rate being 1.0 ml·min^-1 and the detection wavelength at 229 nm. RESULTS The calibration curve was linear in the range of 0. 304-1.520μg （r= 0. 999 9）. The average recovery was 99. 89% （RSD = 1.08% ）. CONCLUSION The mothod is simple , fast and accurate for analysis of prinsepiol-4-O-β-D-glucoside in Valeriana officinalis L.