采用SRAP分子标记技术,对8份野生和8份栽培桔梗种质资源进行遗传多样性研究,旨在为桔梗的遗传育种提供理论依据。结果表明:12对随机引物组合共扩增出109奈谱带,平均每对引物组合扩增出9.1条谱带,其中扩增出80条多态性谱带,平均每对引物组合扩增出6.7条多态性谱带,多态率为73.4%,显示出了较高的多态性。试验中SRAP数据的遗传相似系数范围为0.56~0.88,并以遗传相似系数0.56为标准,将16份桔梗种质资源分为野生桔梗和栽培桔梗二大类群。
Sequence related amplified polymorphis(SRAP)molecular markers were used to detect the genetic diversity of 8 samples of the wild Playtcodon grandiflourus and 8 samples of the cultivation Playtcodon grandiflourus. The purpose of provide the basis for breedong of Playtcodon grandiflourus. The results showed that Twelve primer pairs produced 109 bands,veraged 9. 1 bands per primenr pair,and produced 80 polyrnorphic bands,veraged 6.7 polymorphic bands per primenr pair, showed a higher rate of polymorphic(73. 4%). The SRAP data obtained genetic similarity coefficient range 0. 56~0. 88, the results showed that the 16 samples of Platycodon grandiflorum I3C. were clustered into the wild Playtcodon grandiflourus and the cultivation Playtcodon grandiflourus, two groups at genetic distance 0. 56.