中文摘要：

目的探讨补锌对糖尿病大鼠肝脏糖脂代谢相关基因蛋白磷酸酶2A（PP2A）和磷脂酶D（PLD）表达的调控。方法对补锌糖尿病大鼠差异显示的基因片段进行克隆、测序及同源性分析；设计基因序列引物，RT—PCR检测观察各组大鼠肝脏中基因表达的变化。结果结果显示，片段Zn-4、Zn-8的碱基序列分别与PLD、PP2A同源性为100％，99％。糖尿病对照组PP2AmRNA表达量（0．5072±0．0574）低于正常对照组（1．3303±0．1855），P〈0．05；糖尿病补锌组PP2AmRNA的表达量（0．7005±0．1563）与糖尿病对照组比较有所恢复（P〈0．05）。糖尿病对照组PLDmRNA表达量（1．0754±0．0312）和糖尿病补锌组PLDmRNA的表达量（1．0130±0．0992）均高于正常对照组（0．7995±0．1040），P〈0、05；糖尿病补锌组PLDmRNA的表达量与糖尿病对照组比较无变化（P〉0．05）。结论补锌对糖尿病大鼠肝脏PP2AmRNA表达有上调作用，这可能是锌改善糖尿病糖、脂代谢紊乱的分子机制之一。

英文摘要：

Objective To study the effects of zinc supplementation on related gene expression of liver in diabetic rats. Methods cDNA fragments from former research project were cloned, sequenced and BLASTn analyzed. The RT - PCRs were made using the primers designed according to the sequence of cDNA. Results The identities between the sequence of Zn - 8 and PP2A was 99%. The expression level of DM group （0. 5072 ± 0. 0574） was obviously lower than those of normal group（ 1. 3303 ± 0. 1855, P 〈 0.05）. And The expression level of DM ＋ Zn group（0. 7005 ± 0. 1563） had some improvement compared with those of DM group（P〈0.05）.Conclusion Zinc supplementation can improve the expression of PP2A mRNA on liver in diabetic rats. It may be one of the molecular mechanisms that zinc can improve the disorders of glucose and lipids metabolism in diabetic rats.