欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
近20年来,随着微流控芯片加工技术的不断发展,微流控分析已从一个概念发展为当前世界上最前沿的科技领域之一,微流控芯片上免疫分析的方法研究也取得重要进展。这些芯片包含传输流体的微通道和免疫分析程序中部分或全部的必要组件。微流控技术用于免疫分析在减少试剂用量、缩短分析时间、自动化等方面提高了分析性能。本文综述了微流控芯片上免疫分析的发展、分类,并评述了各类微流控免疫分析芯片的性能及优缺点。
英文摘要：
With the continuous development in microfluidic fabrication technology, microfluidic analysis has evolved from a concept to one of research frontiers in last twenty years. The immunoassay methods based on microfluidic devices have also made great progress. Such devices consist of microchannels for transporting fluids integrated with part or all of the necessary components of an imrnunoassay procedure. Mierofluidic technology improves greatly the analytical performance of immunoassays by reducing the consumption of reagents, decreasing the analysis time, and developing automation. This review focuses on the development and classification of microfluidic immnnoassays, and the characteristics of each type of chips and their performances are also discussed.
同期刊论文项目
同项目期刊论文
Theoretical study of the interaction pattern and the binding affinity between procaine and DNA bases
Effect of enteral nutrition formula on fat absorption and serum free fatty acid profiles in rat with
Zirconia nanoparticles enhanced grafted collagen tri-helix scaffold for unmediated biosensing of hyd
Simultaneous determination of ethamsylate, tramadol and lidocaine in human urine by capillary electr
Novel coupling mechanism-based imaging approach to scanning electrochemical microscopy for probing t
Off-line form of the Michaelis-Menten equation for studying the reaction kinetics in a polymer micro
Microchannel-electrode alignment and separation parameters comparison in microchip capillary electro
Synthesis of metallic nanoparticles protected with N,N,N-trimethyl chitosan chloride via a relativel
Gold nanoparticles doped three-dimensional sol-gel matrix for amperometric human chorionic gonadotro
Simultaneous determination of psychotropic drugs in human urine by capillary electrophoresis with el
Flow-injection chemiluminescent immunoassay for alpha-fetoprotein based on epoxysilane modified glas
Electrogenerated chemiluminescence detection of amino acids based on precolumn derivatization couple
Multilayer membranes for glucose biosensing via layer-by-layer assembly of multiwall carbon nanotube
A gold nanoparticles/sol-gel composite architecture for encapsulation of immunoconjugate for reagent
Simultaneous electrochemiluminescence determination of sulpiride and tiapride by capillary electroph
A disposable electrochemical immunosensor for flow injection immunoassay of carcinoembryonic antigen
Electrochemical immunoassay of human chorionic gonadotrophin based on its immobilization in gold nan
Electrochemical study on synergistic effect of the blending of nano TiO2 and PLA polymer on the inte
Synergistic enhancement effect of magnetic nanoparticles on anticancer drug accumulation in cancer c
Noncovalent axial assembly of Picket-fence porphyrin on nitrogen-doped carbon nanotubes for highly e
Pretreatment-free fast ultraviolet detection of melamine in milk products with a disposable microflu
Determination, characterization and cytotoxicity on HELF of ZnO nanoparticles as ultraviolet screens
Simultaneous fabrication of through-hole porous membrane and nanotubes array in a single step anodiz
New anthracene–tetrathiafulvalene derivative-encapsulated SWNT nanocomposite and its application for
Quantum dot-functionalized poly(styrene-co-acrylic acid) microbeads: stepwise self-assembly, charact
Streptavidin-Functionalized Three-Dimensional Ordered Nanoporous Silica Film for Highly Efficient Ch
Dendritic silver/silicon dioxide nanocomposite modified electrodes for electrochemical sensing of hy
A chemiluminescent immunosensor based on antibody immobilized carboxylic resin beads coupled with mi
A channel-resolved approach coupled with magnet-captured technique for multianalyte chemiluminescent
Multipoint quantification of multimarker genes in peripheral blood and micrometastasis characteristi
Electrochemical study of the effect of functionalized nickel nanoparticles on cellular uptake of leu
Blends of TiO2 nanoparticles and poly (N-isopropylacrylamide)-co-polystyrene nanofibers as a means t
Channel-resolved multianalyte immunosensing system for flow-through chemiluminescent detection of al
A simple electrochemical method for the determination of hydroxyl free radicals without separation p
Electrochemical deposition and mechanism investigation of Prussian blue on graphic carbon paste elec
Surface electric field manipulation of the adsorption kinetics and biocatalytic properties of cytoch
Electrochemical determination of NDPhA via its electrocatalysis at porous Au electrode in room tempe
Improved separation efficiency of neurotransmitters on a native printed capillary electrophoresis mi
Electrochemically deposited nanocomposite film of CS-Fc/Au NPs/GOx for glucose biosensor application
Determination of nitrite based on its quenching effect on anodic electrochemiluminescence of CdSe qu
Highly sensitive amperometric biosensors for phenols based on polyaniline-ionic liquid-carbon nanofi
Direct Electrochemistry of Hemoglobin Immobilized on Colloidal Gold-Hydroxyapatite Nanocomposite for
Functionalization of Carbon Nanotubes with Water-Insoluble Porphyrin in Ionic Liquid: Direct Electro
Composition and Shape Control in the Construction of Functional Nickel Hexacyanoferrate Nanointerfac
One-step pyrolysis method for the synthesis of highly efficient 3D hollow carbon nanostructure suppo
Enhanced electrochemiluminescence efficiency of Ru(II) derivative covalently linked carbon nanotubes
A simple method for fabrication of sole composition nickel hexacyanoferrate modified electrode and i
Effect of surface microstructures on the separation efficiency of neurotransmitters on a direct-prin
Direct electrochemistry and electrocatalysis of hemoglobin on nanostructured gold colloid-silk fibro
Highly sensitive detection of daunorubicin based on carbon nanotubes–drug supramolecular interaction
Anodic electrochemiluminescence of CdSe nanoparticles coreacted with tertiary amine and halide induc
Addition of ethynylferrocene to transition-metal complexes containing a chelating 1,2-dicarba-closo-
Rapid identification and high sensitive detection of cancer cells on the gold nanoparticle interface
Study on the enhanced cellular uptake effect of daunorubicin on leukemia cells mediated via function
Application of the blending of PNIPAM- co -PS nanofibers with functionalized Au nanoparticles for th
Synergistic effect of functionalized nickel nanoparticles and quercetin on inhibition of the SMMC-77
Highly resolved separation and sensitive amperometric detection of amino acids with an assembled mic
Amperometric glucose sensor based on catalytic reduction of dissolved oxygen at soluble carbon nanof
Accelerated direct electrochemistry of hemoglobin based on hemoglobin-carbon nanotube (Hb-CNT) assem
The application of Fe3O4 nanoparticles in cancer research: A new strategy to inhibit drug resistance
Electrochemical DNA biosensor for the detection of short DNA species of Chronic Myelogenous Leukemia
Study of the nonenzymatic glucose sensor based on highly dispersed Pt nanoparticles supported on car
Functional multiwalled carbon nanotube nanocomposite with iron picket-fence porphyrin and its electr
Anodic electrochemiluminescence of CdTe quantum dots and its energy transfer for detection of catech
A disposable two-throughput electrochemical immunosensor chip for simultaneous multianalyte detennin
Enzyme-quantum dots architecture for highly sensitive electrochemiluminescence biosensing of oxidase
Detection of NADH and ethanol based on catalytic activity of soluble carbon nanofiber with low overp
A disposable multianalyte electrochemical immunosensor array for automated simultaneous determinatio
Sensitive reagentless electrochemical immunosensor based on an ormosil sol-gel membrane for human ch
An amperometric immunosensor for separation-free immunoassay of CA125 based on its covalent immobili
A disposable impedance sensor for electrochemical study and monitoring of adhesion and proliferation
One-step immobilization of glucose oxidase in a silica matrix on a Pt electrode by an electrochemica
Simultaneous electrochemiluminescence detection of anisodamine, atropine, and scopolamine in Flos da
Amperometric biosensor for hydrogen peroxide based on myoglobin doped multiwalled carbon nanotube en
Circulating tumor cells in perioperative esophageal cancer patients: Quantitative assay system and p
Highly sensitive flow injection detection of hydrogen peroxide with high throughput using a carbon n
A bio-inspired support of gold nanoparticles-chitosan nanocomposites gel for immobilization and elec
Reagentless biosensor for hydrogen peroxide based on immobilization of protein in zirconia nanoparti
Conductive mesocellular silica-carbon nanocomposite foams for immobilization, direct electrochemistr
Immobilization and electrochemical behavior of gold nanoparticles modified leukemia K562 cells and a
Hydrogen peroxide biosensor based on hemoglobin modified zirconia nanoparticles-grafted collagen mat
Highly efficient and of selective enrichment phosphopeptides using porous anodic alumina membrane fo
The synergistic effect of Prussian-Blue-grafted carbon nanotube/poly(4-vinylpyridine) composites for
Hydrogen bubble dynamic template synthesis of porous gold for nonenzymatic electrochemical detection
Superhydrophobicity of 3D porous copper films prepared by using the hydrogen bubble dynamic template
CdS Quantum dots/ Ru(bpy)32+ electrochemiluminescence resonance energy transfer system for sensitive
Graphene-assisted dual amplification strategy for the fabrication of sensitive amperometric immunose
High Electron Transfer Efficiency of Titania Dioxide Nanotube for Low Potential Electrochemiluminesc
Synthesis of gelatin-stabilized gold nanoarticles and assembly of carboxylic single-walled carbon na
Near infrared sensing based on fluorescence resonance energy transfer between Mn,CdTe quantum dots a
Synthesis, Characterization of Multiangular Nickel Nanoparticles and its Photocatalytic Properties a
Glycoside modification of oleanolic acid derivatives as a novel class of anti-osteoclast formation a
Characterization, direct electrochemistry and amperometric biosensing of graphene by noncovalent fun
Interconnected nanochannel networks of colloidal crystals integrated on a microfluidic chip for high
Quinoxaline derivative of oleanolic acid inhibits osteoclastic bone resorption and prevents ovariect
Room-Temperature Phosphorescence Chemosensor and Rayleigh Scattering Chemodosimeter Dual-Recognition
Electrochemiluminescence analysis of folate receoptors on cell membrane with on-chip bipolar electro
CdS nanoparticles gunctionalized colloidal carbon nanoparticles, preparation, characterization and a
Total contents and fractionation of heavy metals in road deposited sediments collected from differen
Surface-plasmon field-enhanced electrochemiluminescence from CdS thin films by gold nanoparticles fo
Green and facile synthesis of highly biocompatible graphene nanosheets and its application for cellu
Fabrication of a dispersible graphene/gold nanoclusters hybrid and its potential application in elec
Synchronous detection of capacitance and impedance for cell counting and discrimination on a microfl
Fabrication of Graphene-Quantum Dots Composites for Sensitive Electrogenerated Chemiluminescence Imm
Enhanced solid-state electrogenerated chemiluminescence of Au/CdS nanocomposite and its sensing to H
Cytosensor constructed with a biomimetic fibronectin-functionalized carbon nanotubes on glassy carbo
Identification and Quantification of Related Impurities in 2-Chloroethyl Phenyl Sulfide for Industri
Multi-parameter detection of diabetes mellitus on multichannel poly(dimethylsiloxane) analytical chi
Quenching of the Electrochemiluminescence of CdS,Mn Nanocrystals by CdTe Quantum Dots and Its Potent
Simultaneous multiple enantioseparation with one-pot imprinted microfluidic channel by microchip cap
Synthesis and Characterization of High Fluorescence, Type-II Core/Shell CdTe/CdSe Quantum Dots in Aq
Determination of n-octanol/water partition coefficient for DDT-related compounds by RP-HPLC with a n
An example of quality control of fine chemical intermediates: related impurity analysis of industria
A simple fluorescent strategy for in situ evaluation of cell surface carbohydrate with a quantum dot
A novel amperometric biosensor based on gold nanoparticles-mesoporous silica composite for biosensin
Selective detection of hypertoxic organophosphates pesticides via PDMS composite based acetylcholine
A non-element-enriched, non-lyophilized candidate rat serum reference material prepared for once use
Synthesis and evaluation of a novel series of heterocyclic oleanolic acid derivatives with anti-oste
Theoretical investigation on the influence of temperature and crystallographic orientation on the br
Chromatographic retention prediction and octanol-water partition coefficient determination of monoba
Determination of low-level ink photoinitiator residues in packaged milk by solid-phase extraction an
Homogenous Thionine-SiO2 Nanocomposite Spheres: Sonochemical Preparation, Characterization, and Appl
Separation and identification of relative substances in polyurethane chain extender hydroquinone bis
Characterization, Direct Electrochemistry, and Amperometric Biosensing of Graphene by Noncovalent Fu
Low-Potential Electrochemiluminescent Sensing Based on Surface Unpassivation of CdTe Quantum Dots an
Electrochemiluminescent biosensing of carbohydrate-functionalized CdS nanocomposites for in situ lab
Molecularly imprinted magnetic nanoparticles as tunable stationary phase located in microfluidic cha
Cytosensing and dynamic monitoring of cell surface carbohydrate expression by electrochemiluminescen
Noncovalent Assembly of Picket-Fence Porphyrins on Nitrogen-Doped Carbon Nanotubes for Highly Effici
Quantum-Dot-Functionalized Poly(styrene-co-acrylic acid) Microbeads: Step-Wise Self-Assembly, Charac
Fluorescence Resonance Energy Transfer between Quantum Dots and Graphene Oxide for Sensing Biomolecu
In Situ Scanometric Assay of Cell Surface Carbohydrate by Glyconanoparticle-Aggregation-Regulated Si
Surface trap of quantum dots by bidentate chelation for low-potential electrochemiluminescent biosen
Real-time monitoring of cell viability by its nanoscale height change with oxygen reduction as endog
Ultraviolet detection of amino acids based on their on-column conjugation with cupric cation using a
In Situ Electrochemical Imaging of Membrane Glycan Expression on Micropatterned Adherent Single Cell
Pt-dispersed flower-like carbon nanosheet aggregation for low-overpotential electrochemical biosensi
Lectin-Based Nanoprobes Functionalized with Enzyme for Highly Sensitive Electrochemical Monitoring o
A Reusable Interface Constructed by 3-Aminophenylboronic Acid Functionalized Multiwalled Carbon Nano
In situ scanometric assay of cell surface carbohydrate by glyconanoparticle-aggregation regulated si
Quantum Dots Based Electrochemiluminescent Immunosensor by Coupling Enzymatic Amplification with Sel
Selective Detection of p-Phenylenediamine in Hair Dyes Based on a Special CE Mechanism Using MnO2 Na
Transfer of carbohydrate expression information on cell-adhered surface to a secondary surface by co
Electrochemiluminescence detection of near single DNA molecule by using quantum dots-dendrimer nanoc
Synthesis of prussian blue nanocrystals with controlled properties by a polysaccharide hydrolyzation
Gold nanoparticles-coated magnetic microspheres as affinity matrix for detection of hemoglobin A1c i
Electrochemical sensing for caspase 3 activity and inhibition using quantum dot functionalized carbo
Highly sensitive fluorescent analysis of dynamic glycan expression on living cells using glyconanopa
Amplified electrochemiluminescence of quantum dots by electrochemically reduced graphene oxide for n
Functionalization of graphene nanoribbons with porphyrin for electrocatalysis and amperometric biose
Convenient enantioseparation by monolithic imprinted capillary clamped in a chip with electrochemica
Carbon nanospheres enhanced electrochemiluminescence of CdS quantum dots for biosensing of hypoxanth
Ultrasensitive scanometric strategy for detection of matrix metalloproteinases using a histidine tag
Signal amplification by adsorption-induced catalytic reduction of dissolved oxygen on nitrogen-doped
Simultaneous multiple enantioseparation with a one-pot imprinted microfluidic channel by microchip c
Visual Scanometric Detection of DNA through Silver Enhancement Regulated by Gold-Nanoparticle Aggreg
Ultrasensitive Immunoassay of Protein Biomarker Based on Electrochemiluminescent Quenching of Quantu
Electrochemiluminescence detection of near single DNA molecules by using quantum dots-dendrimer nano
Streptavidin-functionalized capillary as an immunosensor for highly efficient chemiluminescent immun
The use of polyethylenimine-grafted graphene nanoribbon for cellular delivery of locked nucleic acid
Ultrasensitive Multiplexed Immunoassay with Electrochemical Stripping Analysis of Silver Nanoparticl
High quantum transfer efficiency of titania dioxide nanotube for low potential electrogenerated chem
Dissemination profile of perioperative tumor cells in peripheral blood of colorectal cancer patients
A label-free strategy for facile electrochemical analysis of dynamic glycan expression on living cel
A glucose biosensor based on direct electrochemistry of glucose oxidase immobilized on nitrogen-dope
Sandwich nanohybrid of single-walled carbon nanohorns-TiO2-porphyrin for electrocatalysis and ampero
A rapid and simple method for ultrasensitive electrochemical immunoassay of protein by an electric f
Disposable microfluidic device with ultraviolet detection for highly resolved screening of illicit d
Dual Signal Amplification of Glucose Oxidase-Functionalized Nanocomposites as a Trace Label for Ultr
Three-Minute-Long Chemiluminescent Immunoassay Using Dually Accelerated Immunoreaction by Infrared H
Disposable electrochemiluminescent biosensor using bidentate-chelated CdTe quantum dots as emitter f
Fabrication of tunable microreactor with enzyme modified magnetic nanoparticles for microfluidic ele
FAST AND HIGH-PERFORMANCE SCREENING OF NARCOTIC DRUGS ON A MICROFLUIDIC DEVICE BY MICELLAR ELECTROKI
Amperometric sensor for ethanol based on one-step electropolymerization of thionine-carbon nanofiber
Al2O3/SnO2Co-nanoparticle modified grafted collagen for improving thermal stability and infrared emi
The effect of UV irradiation on the binding of anticancer drug doxorubicin to DNA in the presence of
DNA binding behavior of doxorubicin based on photovoltaic effect of nano titanium dioxide modified e
The enhancement effect of gold nanoparticles to facilitate the drug delivery and biomarker of drug-r
Magnetic controllable biorecognition process of doxorubicin detected by electrochemical contact angl
Amperometric detection of carbohydrates with a portable silicone/quartz capillary microchip by desig
Electrochemiluminescence sensors for scavengers of hydroxyl radical based on its annihilation in CdS
期刊信息
位置：