中文摘要：

目的观察恒磁场（CMF）对C反应蛋白（CRP）作用下大鼠骨髓来源内皮祖细胞（EPC）增殖、凋亡及一氧化氮（NO）分泌的影响。方法密度梯度离心法获得SD雄性大鼠的骨髓EPC，无菌条件下培养4d；实验分为3组：空白对照组、CRP（12μg／ml）组及CRP＋CMF（0．1mT、0．5mT、1．0mT）组。各组皆于24h后收集标本，MTT法检测细胞增殖能力，流式细胞仪检测细胞凋亡率，硝酸还原酶法检测培养液中的NO含量。结果与对照组相比，CRP组细胞增殖（吸光度）显著降低（P〈0．05），NO分泌量显著降低（P〈0．05），细胞凋亡率显著增加（P〈0．05）；CRP＋0．5mT和1．0mT CMF组细胞增殖吸光度显著高于CRP组（P〈0．05），NO分泌量显著高于CRP组（P〈0．05），细胞凋亡率显著低于CRP组（P〈0．05）。结论0．5mT和1．0mT CMF可拮抗CRP的作用，促进EPC的增殖及NO分泌并抑制EPC凋亡。

英文摘要：

Objective To investigate the effects of constant magnetic field （CMF） on proliferation, apoptosis and nitric oxide （NO） secretion of rat bone marrow-derived endothelial progenitor cells （EPCs） intervened by C- reactive protein （CRP）. Methods EPCs were isolated from rat bone marrow by density gradient centrifugation and cultured on fibronectin-coated dishes. The cells were divided into five groups, i. e., control group, CRP （ 12 μg/ml） group, CRP plus CMF （0.1 , 0.5, 1.0 mT） groups. Samples were collected 24 hours after incubation. Cell proliferation was measured by MTT chromatometry. Apoptosis rate was detected by flow-cytometry. NO content of culture medium was measured by nitrate reductase method. Results As compared with control group, cell prolifer- ation in CRP group reduced significantly （0,265 ±0. 008 vs 0,316 ± 0,011, P 〈 0.0.5 ） , NO secretion also decreased significantly [ （22.7 ± 4.5 ） μmol/L vs （37.6 ± 5.8 ） p, mol/L, P 〈 0. 05 ] , cell apoptosis rate elevated significantly [（10.8±0.8）% vs （4.2±0.5）%,P〈0.051. Cell proliferation in CRP plus0. 5 mT or 1.0 mT CMF group （0. 295 ± 0. 009,0. 302 ± 0. 010） were much more than those in CRP group （P 〈0.05 ） ,NO secretion contents [ （28.3 ± 4.9 ） μmol/L, （29.2 ±5.6 ） μmol/L ] were also much mere than those in CRP group （ P 〈 0.05 ） , apoptosis rate [ （7.4 ± 0.5） % , （6.9 ± 0.6） % ] was significantly lower than that in CRP group （P 〈 0.05 ）. Conclusion CMF at intensity of 0.5 mT and 1.0 mT can antagonize the effects of CR, promote proliferation of EPCs and secretion of NO and inhibit apoptosis rate of EPCs.