1引言 沙拉沙星(SARA)是继恩诺沙星之后又一种新的动物专用氟喹诺酮类药物,是防治畜禽疾病的理想药物。目前,测定SARA的方法主要有高效液相色谱法、荧光分析法和毛细管电泳串联质谱法等。
A spectrofluorimetric method for the determination of Sarafloxacin (SARA) based on the fluorescence enhancement of Tb3+-SARA complex in the presence of sodium dodecyl sulfate (SDS) and boric acid was developed. The effect of various experimental parameters, such as pH value, the type and amount of surfactant, the type and amount of buffer solution and the concentration of Tb3+, on the fluorescence intensity of the complex was investigated. Under the optimum conditions, the enhanced fluorescence intensity of the system showed a good linear relationship with the concentration of SARA in the range of 0. 100-1.80 × 103 μg/L with a correlation coefficient of 0.9994. The detection limit of SARA was found to be O. 02 μg/L, and relative standard deviation (RSD) was 1.8% (c= 10.0 μg/L, n= 11 ). The method has been successfully applied to the determination of SARA in milk samples with the recoveries of 95.5%-101%.