<正>Genome sequencing of more than 80 thermophiles has provided powerful databases for the preliminary determination of genes essential to thermal adaptation(Berka et al.,2003;Berezovsky and Shakhnovich,2005;Andrews et al.,2010).A laboratory strain of Thermoanaerobacter tengcongensis MB4 has been shown to grow at temperatures over the range
Genome sequencing of more than 80 thermophiles has pro- vided powerful databases for the preliminary determination of genes essential to thermal adaptation (Berka et al., 2003; Berezovsky and Shakhnovich, 2005; Andrews et al., 2010). A laboratory strain of Thermoanaerobacter tengcongensis MB4 has been shown to grow at temperatures over the range 50℃ to 80℃, with an optimum of 75℃ (Xue et al., 2001) and its thermal adaptation mechanisms have been studied with the sequencing of its genome (Bao et al., 2002). Using two- dimensional gel electrophoresis (2DE) and Matrix-Assisted Laser Desorption/Ionization Time of Flight (MALDI-TOF/ TOF) mass spectrometry, Wang et al.