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中文摘要：
将八肋游仆虫第一类肽链释放因子基因eRF1a克隆入表达载体pRSETc,并在大肠杆菌Bl21（DE3）中进行诱导表达.表达形成的包含体经切胶回收,然后免疫大鼠制备抗血清.所获得的抗血清用Amersham Biosciences抗体纯化试剂盒进行纯化,SDS-PAGE电泳分析表明纯化后的抗体纯度很高.Western blotting印迹和ELISA鉴定结果表明抗体特异性好,效价为1∶5000.
英文摘要：
Euplotes octocarinatus eRF1a gene was cloned into the expression vector pRSETc. The obtained plasmid pRSETC-eRF1a was transformed into E. coli BL21 （DE3）,and the fusion protein His6-eRF1a was expressed. The wistar rat was injected with purified His6-eRF1a inclusion bodies to induce the immunoreactions. The antiserum was purified with Mab Trap Kit and detected by SDS-PAGE,ELISA and Western blotting. The results showed that the polyclonal antibody had high purity and specificity. The antibody titer detected by ELISA reached to 1 ： 5 000.
同期刊论文项目
同项目期刊论文
Identification of translational release factor eRF1a binding sites on eRF3 in Euplotes octocarinatus
Polyclonal antibody against a recombinant chlorotoxin-like peptide from the Chinese scorpion and det
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