运用普适校正原理,不需要Mark常数K、α值,使用示差折光检测仪及凝胶渗透色谱系统,直接测定了葡聚糖的相对分子质量及相对分子质量分布,优化了色谱操作条件,证明凝胶渗透色谱是监控淀粉酶解反应程度的一种有效手段.
The molecular weight and distribution of dextran was detected directly by gel permeation chromatography (GPC) and differential refractive detector, which is modified with the theory of adjustability emendation. In this method the operation conditions were optimized and do not need to know the constant of Mark and α. So the GPC could be an effective measure to control the degree of starch hydrolysis reaction the value of K in time.