中文摘要：

【目的】探讨中药狼疮Ⅱ号对缺乏淋巴增殖基因（MRL／lpr）狼疮鼠Th1／Th2相关细胞因子谱的影响。【方法】选用自发性系统性红斑狼疮（SLE）模型MRL／lpr狼疮鼠40只，随机分为空白对照组、狼疮Ⅱ号组（中药组，剂量为20g·kg^-1·d^-1）、强的松组（西药组，剂量为6mg·kg^-1·-d^-1）、中西结合组（20g·kg^-1·d^-1狼疮Ⅱ号＋6mg·kg^-1·d^-1强的松）；连续用药8周后，采用Flowcytomix流式检测技术对血清中多种Th1／Th2细胞因子进行芯片式集成测定。【结果】与空白对照组比较，中西结合组粒细胞-巨噬细胞集落刺激因子（GM—CSF）水平显著降低（P〈0．05）；各治疗组干扰素-γ（IFN-γ）、肿瘤坏死因子-α（TNF—α）水平显著降低，且中西结合组两因子水平显著低于单纯中药组及西药组（P〈0．05或P〈0．01）；各治疗组白介素-2（IL-）水平显著升高，IL—17水平显著降低（P〈0．05或P〈0．01），但3组间比较差异无显著性意义（P〉0．05）；各治疗组IL-4、IL-5、IL-10水平显著降低，且中西结合组IL—10水平显著低于单纯中药组和西药组（P〈0．05或P〈0．01）；西药组和中西结合组IL石水平显著降低（P〈0．01），且中西结合组IL-6水平显著低于西药组（P〈0．05）。【结论】狼疮Ⅱ号可能通过调节Th1／Th2细胞因子网络的平衡，影响B细胞、T细胞过度活化而发挥治疗SLE的作用．

英文摘要：

Objective To study the influence of Langchuang Ⅱ, a Chinese medicinal compound formula, on Th1/TH2 cytokines of MRL/lpr lupus mice. Methods Forty MRL/lpr mice with spontaneous systemic lupus erythematosus （SLE） were randomized into blank control group, Langchuang Ⅱ group （in the dose of 20 g · kg^-1 · d^-1）, prednisone group （ in the dose of 6 mg · kg^-1 · d^-1 ）, and the combination group （ Langchuang Ⅱ and prednisone）. After medication for 8 continuous weeks, Flowcytomix technology was used for the detection of different kinds of serum Th1/Th2 cytokines. Results Compared with the blank control group, serum granulocyte-macrophage colony-forming unit （GM-CSF） level was decreased in the combination group （ P 〈 0. 05 ）, interferon γ （IFN-γ） and tumor necrosis factor α （TNF-α） were lowered in the three medication groups, and the decrease was obvious in the combination group （P 〈0. 05 or P 〈 0. 01 ） ; interleukin-2 （IL-2） was increased and IL-7 was decreased in the three medication groups （ P 〈 0.05 or P 〈 0. 01 ）, but the differences were insignificant between the three groups （P 〉0. 05）; IL-4, IL-5 and IL-10 were all decreaed in the medication groups, and the decrease of IL-10 was obvious in the combination group （P 〈 0. 05 or P 〈 0. 01 ） ; IL-6 was lower in prednisone group and the combination group than that in the blank control group （P 〈 0. 01 ）, and was the lowest in the combination group （P 〈0. 05 ）. Conclusion The therapeutic mechansim of Langchuang Ⅱ for SLE is probably related with the inhibition of hyperactivation of B cells and T cells through regulating the balance of Th1/Th2 cytokines.