中文摘要：

目的检测EB病毒转化前后人类B细胞中与IgA1糖基化相关基因ST6GALNAC2的表达以及其所翻译的α2，6．唾液酸转移酶效应的改变情况，探讨EB病毒转化后的B淋巴细胞能否完全替代新鲜外周血中提取的B淋巴细胞。方法IgA肾病患者2例，IgA骨髓瘤患者2例和健康对照3例为研究对象。用CD19免疫磁珠提取研究对象新鲜外周血B淋巴细胞，并用EB病毒转化获得永生化B淋巴细胞。实时定量荧光PCR法检测新鲜外周血B淋巴细胞和EB病毒转化后的B淋巴细胞中ST6GALNAC2基因的表达。用EHSA法检测外周血清和EB病毒转化后B淋巴细胞培养上清液中IgA1分子上唾液酸水平。结果EB病毒转化后的B淋巴细胞较外周血中新鲜提取的B淋巴细胞ST6GALNAC2基因表达和分泌的IgA1分子上唾液酸水平均明显下降。结论当研究糖基化相关基因的表达及其编码的糖基化相关酶的效应时，EB病毒转化后的B淋巴细胞不能完全替代新鲜外周血中提取的B淋巴细胞。

英文摘要：

Objective To investigate the influences of Epstein-Barr virus transformation on the expression of IgA1 molecular glycosylation associated gene ST6GALNAC2 and the alteration of its encoded protein 2,6-sialyhransferase. Methods Two patients with IgA nephropathy, 2 patients with IgA multiple myeloma and 3 healthy controls were enrolled in the study. Peripheral blood samples from participants were used for B lymphocytes enrichment by CD19^＋ immunomagnetic beads and for EBV transformation. The expression of gene ST6GALNAC2 was detected by real-time RT-PCR and the level of sialic acid in IgA1 molecule was observed by ELISA. Results The expression level of gene ST6GALNAC2 in B lymphocytes after EBV transformation was lower than that enriched from fresh peripheral blood. The level of sialic acid in IgA1 molecule secreted from B lymphocyte was down-regulated after EBV transformation. Conclusion In the investigations on the expression of IgA1 molecular glycosylation associated genes and the effect of its encoded enzymes, B lymphocytes enriched from fresh peripheral blood can not be entirely replaced by B lymphocyte transformed by EBV.