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中文摘要：
选用垂盆草2-4mm带腋芽茎段作为包埋外植体,研究海藻酸钠浓度、激素配比、活性炭、离子交换时间及人工种子培养条件对人工种子成型、萌发及成苗的影响,探索适合垂盆草的人工种子制作方法。结果表明,采用滴珠法,以MS＋4%海藻酸钠＋1.0mg·L^-1 6-BA＋0.1mg·L^-1GA3＋0.5mg·L^-1 NAA为人工胚乳,与2%CaCl2水溶液发生离子交换反应15min后获得的人工种子呈规则的球形、富弹性,在MS培养基中培养20d萌发率达95%,30d生根率达90%,在培养皿中催芽成苗率达60%,为制备垂盆草人工种子的适宜方法。
英文摘要：
Effects of synthetic endosperm components, activated carbon and germination conditions on the germination and rooting rate of artificial seeds of Sedum sarmentosum were researched by using 2-4 mm shoot cuttings with the buds used as propagules. The optimal medium used as artificial endosperm was MS ＋4% sodium alginate＋1. 0 mg · L^-1 6-BA＋0.1 mg· L^-1 GA3 ＋0.5 mg· L^-1 NAA. The spherical and elastic artificial seeds were prepared after dropping the shoot cuttings into the solution of 2% CaCI2 for 15 min. The germination rate of the artificial seeds was 95% after culture for 20 days in MS medium,the rooting rate was 90% after 30 days,and the seedling rate was 60% in a Petri dish germination. This formula was a suitable combination of artificial seeds for S. sarmentosum.
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