中文摘要：

目的：研究儿茶酚异喹啉类物质1-甲基-6,7-二羟基-1,2,3,4-四氢异喹啉（salsolinol,Sal）对帕金森病关键蛋白α突触核蛋白（α-syulcein,α-syn）纤维化程度的影响。方法：将重组人源α-Syn蛋白与不同浓度的Sal共同孵育,通过ThT染色对α-Syn成纤维状况进行监测。同时,利用扫描电镜和激光共聚焦显微镜观察α-Syn与Sal共孵育后的形态变化。结果／结论：实验结果表明Sal具有将α-Syn稳定在寡聚体或者原纤维状态的能力,且呈浓度依赖性,Sal浓度越大,α-Syn纤维化进程越缓慢。这一发现为进一步研究α-Syn和儿茶酚异喹啉类物质在PD发生和发展过程中起到的作用提供了一定的实验依据。

英文摘要：

Object This study was to explore the relationship between salsolinal（Sal） and the fi bration of α-synuclein（α-Syn）. Method Recombinant humana α-Syn was incubated with different concentration of Sal, respectively. The fi bration of α-syn was investigated via thionfl avin T（thT） assay, confocal laser scanning microscopy and transmission electron microscopy（TEM）. Results／Conclusion Consequently, it was found that Sal could able to stabilize α-Syn in the oligomer form or protofi brils, which was more toxic than the fi ber form. This result provides new evidence for understanding the pathogenesis of PD.