中文摘要：

我们的以前的研究显示出那 γ-aminobutyric 酸(伽马氨基丁酸) 和伽马氨基丁酸 _B 受体涉及孕酮或伽马氨基丁酸导致的老鼠精子 acrosome 反应。这里，我们报导伽马氨基丁酸 _{在老鼠睾丸和精子的 C} 受体。编码 ρ1， ρ2 和伽马氨基丁酸 _C 受体从老鼠睾丸被克隆；他们的序列与老鼠伽马氨基丁酸 _{在视网膜的 C} 受体。反向的抄写聚合酶链反应分析证明在老鼠睾丸的开发期间， ρ1 和 ρ2 子单元的抄本层次显示出小变化，当 ρ3 的表达式是逐渐地起来调整的时。用 anti-ρl 抗体的 Immunofluorescence 分析揭示了那伽马氨基丁酸 _C 受体在伸长的 spermatid 和精子上存在。用金霉素试金，我们发现那 N (4 )-chloroacetylcytosine arabinoside，伽马氨基丁酸 _C 受体收缩筋，触发的老鼠精子 acrosome 反应；而(1,2,5,6-tetrahydropyridin-4-yl ) methylphosphinic 酸，伽马氨基丁酸 _C 受体对手，禁止了 N (4 )-chloroacetylcytosine arabinoside 的能力导致 acrosome 反应。这些结果建议了那伽马氨基丁酸 _C 受体也涉及老鼠精子 acrosome 反应。

英文摘要：

Our previous studies showed that γ-aminobutyric acid （GABA）A and GABAB receptors are involved in rat sperm acrosome reaction induced by progesterone or GABA. Here, we report the presence of GABAc receptor in rat testis and spermatozoa. Full-length complementary DNA encoding the ρ1, ρ2 and ρ3 subunits of GABAC receptor were cloned from rat testis; their sequences are identical to those of rat GABAC receptor in retina. Reverse transcription-polymerase chain reaction analysis showed that during the development of rat testis, the transcript levels of the ρ1 and ρ2 subunits showed little change, while the expression of ρ3 was gradually upregulated. Immunofluorescence analysis using an anti-ρ1 antibody revealed that GABAc receptor exists on the elongated spermatid and sperm. Using a chlortetracycline assay, we found that N（4）-chloroacetylcytosine arabinoside, a GABAC receptor agonist, triggered rat sperm acrosome reaction; whereas （1,2,5,6-tetrahydropyridin-4-yl） methylphosphinic acid, a GABAC receptor antagonist, inhibited the ability of N（4）-chloroacetylcytosine arabinoside to induce acrosome reaction. These results suggested that GABAC receptors are also involved in rat sperm acrosome reaction.