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中文摘要：
将固体表面荧光法和荧光偏振测量相结合,直接利用固体粉末进行荧光偏振检测,研究了维生素B2固体粉末的荧光偏振度及其特性。实验结果表明,维生素B2固体粉末的荧光偏振度受激发光入射角影响;当样品粉末紧密度大于0.6667时,紧密度不会对荧光偏振度产生影响。在激发光入射角为45°条件下其荧光偏振度为0.4178。该结果为使用维生素B2水溶液进行荧光偏振检测的结果的1.42倍。对维生素B2固体粉末连续进行6h照射激发,其荧光偏振度检测结果在误差范围内保持稳定。
英文摘要：
Using solid powder directly,an approach to acquire the fluorescence polarization is demonstrated by combining solid surface fluorimetry and fluorescence polarization,and the fluorescence polarization properties of vitamin B2 are researched with this method.The results show that the degree of fluorescence polarization of vitamin B2 solid powder is dependent on the incident angle of the exciting light,and is independent of the relative compactness when it is greater than 0.6667.The degree of polarization hits 0.4178 while the incident angle is 45°.It is 1.42 times of that the detected result from the aqueous solution.The degree of fluorescence polarization of the vitamin B2 solid sample keeps steadily in error band under constant illumination for 6 h.
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