中文摘要：

目的：明确 HPV38E6/E7感染对 HaCaT 细胞增殖和凋亡的影响。方法通过构建 pGCMV和pGCMV-HPV38E6/E7质粒，建立HaCaT稳定转染细胞系并进行鉴定；CCK-8检测细胞增殖；流式细胞仪检测细胞周期及凋亡。结果与对照组相比，转染HPV38E6/E7的HaCaT细胞增殖明显增加，S期细胞增多，凋亡减少，以上差异均具有统计学意义（P＜0.05）。结论 HPV38E6/E7对角质形成细胞增殖及凋亡的影响，可能是β-HPV引起非黑素瘤性皮肤癌发生的重要病理生理学基础。

英文摘要：

Objective On the basis of cell proliferation and apoptosis, to determine the effect of HPV38E6/E7 infection on HaCaT cell line. Methods We established and indentified HPV38E6/E7 stable expression cell strain by constructing the pGCMV and pGCMV-HPV38E6/E7 plasmids;cell proliferation was measured by CCK-8;cell cycle and apoptosis were determined through flow cytometry analysis. Results Compared with control group, after transfected with plasmids HPV38E6/E7, the HaCaT cells show the significantly increase of cell proliferation, S phase and reduce of cells apoptotic, the above differences were statistically significant （P〈0.05）. Conclusions HPV38E6/E7 had a certain effect on the proliferation and apoptosis of keratinocytes, probably due to the fact thatβ-HPV is an important basis of pathophysiological which causes the non-melanoma skin cancer.