中文摘要：

从 EMS 诱变日本晴种子的 M2代中筛选得到一个植株明显矮化、分蘖数急剧增多的突变体,命名为 det1（dwarf and excessively-tillering 1）.det1还显示出明显的穗型、粒型和育性等方面的生殖发育缺陷.遗传分析表明,det1受一对隐性基因控制.以 det1与品种 DZ60杂交建立了 F2定位群体,利用微卫星标记分析F2群体中的突变体,将 DET1基因定位在第6染色体长臂端,标记 SSR2和 SSR3之间约68 kb 的范围内.已知该区间内存在一个与水稻株高和分蘖有关的基因 D3.测序结果表明,DET1与 D3是同一个基因,编码一个 LRR 型的 F-box 蛋白.与 d3相比,det1还显示了新的表型特征,推测可能是等位基因的不同突变位点造成的.det1/d3的表型特征提示,DET1/D3在调节水稻生长发育过程中具有多效性.因此,利用 det1开展进一步的研究将有助于全面揭示 DET1/D3的分子功能.

英文摘要：

A mutant called det1 （dwarf and excessively-tillering 1） was identified in the M2 generation of Nipponbare seeds treated with EMS, which exhibited serious dwarf and an excessive increase in tiller number. The det1 mutant also showed obviously defects in reproductive development including panicle size, grain size and spikelet fertility, etc. Genetic analysis indicated that det1 should be controlled by a single recessive gene. In order to map the gene, an F2 population was constructed by crossing the det1 mutant with variety DZ60 （Oryza sativa L. indica）. By analyz-ing mutant plants in the F2 population using microsatellite markers, DET1 was mapped to a 68 kb region between markers SSR2 and SSR3 on the long arm of chromosome 6 in rice. It has been known that a gene D3 controlling rice height and tillering existed in this region. Sequencing results suggested that DET1 and D3 should be the same gene, which encodes a leucine-rich repeat （LRR） typed F-box protein. Compared with d3, det1 exhibited some ad-ditional mutant phenotypes probably caused by their different mutation sites. The phenotypes of det1/d3 suggested that DET1/D3 should play pleiotropic roles in regulating rice growth and development. Therefore, comprehensive studies on det1 would facilitate complete understanding of the molecular function of DET1/D3.