中文摘要：

目的：探讨参附注射液对心源性休克大鼠血浆中内源性物质的影响。方法：32只SD大鼠随机分为空白组、模型组、阳性对照组和给药组，每组8只。除空白组外，其余各组均采用结扎左冠状动脉法复制大鼠心源性休克模型，阳性对照组给予0．82mg·kg-1多巴胺注射液，给药组给予10mL·kg-1参附注射液，空白组和模型组给予生理盐水。给药45min后。心尖取血，HPLC—MS测定大鼠体内内源性物质含量，采用主成分分析法分析组间差别，正交信号校正和偏最小二乘法判别分析法分析可能的生物标记物。结果：主成分分析结果显示空白组、模型组、对照组和给药组之间无样本点重合。正交信号校正偏最小二乘法分析显示大鼠血浆中肌酸、3-hydroxydodecanoicacid、花生四烯酸、溶血磷脂质等物质在实验各组间发生了相应的变化。结论：参附注射液对心源性休克模型大鼠血浆中内源性物质及其整体状态具有明显的影响，肌酸、精胺、3．hydroxydodecanoicacid、花生四烯酸、溶血磷脂质等可能是潜在生物标记物。

英文摘要：

Objective： To study the effect of Shenfu injection on endogenous subs-tances in plasma of acute cardiogenic shock rats. Method： Thirty-two Sprague-Dawley rats were randomly divided into control group, model group, positive group, administration group, with ten rats in each group. Each group was made by blocking left coronary artery except the control group, the positive group was administrated with Dopamine injection, the administration group was administrated with Shenfu injection. The control group and model group were only administrated with normal saline. Fourty-five minutes later, plasmawas collected from cardiac apex. Liquid chromatography-mass spectrometry was used to analyze the endogenous compounds, principal component analysis （PCA） was used to observe the difference between the four groups, and orthogonal signal correction-partial least square was used to discriminate analysis （OSC-PLS-DA） of biomarkers. Result： In the scatter plot of PCA, there was no samples coincidence among the four group. The further analysis on loading plot of OSC-PLS-DA indicated that creatine, 3-hydroxydodecanoic acid, arachidonic acid, lysophospholipid, etc have changed in the different groups. Conclusion： Shenfu injection has important effect on the endougenous substances in the acute cardiogenic shock rats. Creatine, spermine, 3-hydroxydodecanoicacid, arachidonicacid, lysoph-ospholipid may be the potential biomarkers.