中文摘要：

目的探讨核结合因子a1（Cbfal）基因修饰的兔皮肤成纤维细胞与煅烧骨构建的移植材料对长骨大段骨缺损的修复作用。方法分离、纯化、培养兔皮肤成纤维细胞（RSF），应用pSG5-Cbfal及pSG5质粒分别转染RSF，获得pSG5-Cbfal／RSF及pSG5／RSF，培养生长48h后，分别接种于长2cm、直径4mm的圆柱状牛煅烧骨（TBC）上，制成pSG5-Cbfal／RSF／TBC及pSG5／RSF／TBC复合物材料。制备兔桡骨中部长2cm的骨缺损模型，随机分组后，植入pSG5一Cbfal／RSF／TBC材料作为实验组（I组），以pSG5／RSF／TBC材料植入组（Ⅱ组）、单纯TBC材料植入组（Ⅲ组）和无植入材料的自然修复组（Ⅳ组）作为对照，每组6只（12个桡骨）动物。分别于术后第4周及第12周摄X线片检查并最终获取骨折处标本，对植骨处进行三点抗折弯测试、脱钙骨组织切片的HE染色和Masson染色及新生骨小梁图像计量分析。结果I组标本骨质生长活跃，有大量新生骨小梁，生物力学性能良好，骨缺损处已完成早期愈合及修复；II组、Ⅲ组及Ⅳ组无明显新生骨形成，未显示骨修复现象。结论CbfaI基因修饰的兔皮肤成纤维细胞经与煅烧骨复合植入兔体内后，能完成对大段骨缺损的修复。

英文摘要：

Objective To investigate bone defect healing by true bone ceramic complex carrying core binding factor al （Cbfal） gene modified rabbit skin fibroblasts. Methods Transfect rabbit skin fibroblasts （RSF） with both eukaryotic expression vector pSG5 which could express Cbfal gene and pSG5. After being cultured for 48 h, the transfected RSF were seeded into true bone ceramic （ TBC ） of 2 cm in length and 4 mm in diameter to construct pSG5-Cbfal/RSF/TBC complex and pSG5/RSF/TBC complex. Forty-eight bone defect model rabbits were randomized into four groups, each has 6 rabbits （ 12 radiuses ） , due to different treatment, group I ： with pSG5-Cbfal/RSF/TBC complex, group II ： with pSG5/RSF/TBC complex, group III ： with TBC, GrouplV ： empty control. After being seeded and cultured for about 24 h the complexes were implanted into 2 cm long bone defects in the middle of bilateral radiuses of rabbits. The radiuses were inspected by X-ray and then the specimens were collected at the end of the fourth and twelfth weeks after operation. Then, the specimens were decalcified and histologically investigated with Hematoxylin eosin staining and Masson staining methods. Newly synthesized trabecular bone was inspected by image analysis system and the strength of bone defect area treated with graft-implantation was tested with biomechanical method-three point bending test. Results In group I , trabecular bone was actively synthesized to generate a great amount of trabecular bone and osteon. Preliminary union and bone defect healing were completed with good biomechanical characteristics. There were no newly synthesized trabecular in the other three groups, and bone defect healing were not discovered. The amount of newly synthesized trabecular bone and the results of biomechanical testing differed significantly between group I and the other three（P 〈 0.01 ）. The efficacy of group I was significantly better than that of the other three groups. Conclusion True bone ceramic complex composed with Cbfal gene modif