中文摘要：

目的：了解2012-2013年贵阳市非结核分枝杆菌（NTM）的流行情况。方法：对贵阳市某医院2012-2013年就诊的可疑结核病患者进行分枝杆菌的培养鉴定,采用PCR-荧光探针及基因芯片法进行鉴定,并对相关结果进行分析。结果：1 500例患者经分离培养和PCR-荧光探针及基因芯片法鉴定为结核分枝杆菌感染的1 469例,鉴定为NTM感染的31例,占2.06%,其中8株为鸟分枝杆菌,10株为胞内分枝杆菌,9株为龟或脓肿分枝杆菌,偶然分枝杆菌、瘰疬分枝杆菌、堪萨斯分枝杆菌及蟾蜍分枝杆菌各1株,分别占NTM 25.80%、32.25%、29.03%、3.23%、3.23%、3.23%、3.23%;NTM感染的阳性患者中,男女比例为1.21∶1,30~59岁年龄段占61.29%;不同性别的感染高峰年龄段不同,男性以75~89岁、女性则以45~59岁年龄段。结论：贵阳市存在一定比例的NTM菌感染,以鸟分枝杆菌、胞内分枝杆菌、龟/脓肿分枝杆菌为主。

英文摘要：

Objective：To investigate the prevalence of nontuberculosis mycobacterium（ NTM） in Guiyang city from 2012 to 2013. Methods：NTM culturing and strain identification were adopted in patients with suspected tuberculosis in a hospital of Guiyang during the period from 2012 to 2013,PCR-fluorescent probe and gene chip were adopted for identification and analyzing relevant results.Results：1469 strains were identified as Mycobacterium tuberculosis from 1500 strains of clinical isolated and PCR-fluorescent probe and gene chip method,31 strains were NTM,accounted for 2. 06%.Of which 8 strains were Mycobacterium avium,10 strains were Mycobacterium intracellulare,9 strains were Turtle/Mycobacterium abscessus; while there was only one Mycobacterium fortuitum,Mycobacterium scrofulaceum,Kansas Mycobacterium tuberculosis and Mycobacterium xenopi respectively. The proportion was 25. 80%,32. 25%,29. 03%,3. 23%,3. 23%,3. 23%,3. 23% in NTM. Among the NTM-positive patients,the proportion of male to female was 1. 21：1. Patients at age of 30 ~ 59 was61. 29%. Affection peak age group was different in different genders：male was between 75 to 89 and female was between 45 to 59. Conclusions：There is a certain proportion of NTM affection in Guiyang.Mycobacterium avium,Mycobacterium intracellulare and Turtle/Mycobacterium abscessus were the main pathogenic bacteria.