中文摘要：

为获得高产量纯品植物乳杆菌素PlnE和PlnF,研究其开发生物兽药的可能,试验通过人工合成植物乳杆菌素基因PlnE和PlnF,构建表达载体pGEX-4T-E和pGEX-4T-F,经IPTG诱导表达条件优化后,进行亲和层析纯化融合表达蛋白,并使用SDS-PAGE进行鉴定。试验结果表明,pGEX-4T-E和pGEX-4T-F构建成功,纯化后获得2种产量高达30%的纯品融合蛋白,SDS-PAGE鉴定两融合蛋白表达正确。说明PlnE和PlnF基因片段编码的多肽能在原核细胞中正确表达,为进一步研究该细菌素的生物活性奠定了基础。

英文摘要：

In order to obtain high-yield pure plantaricin,and research on biological veterinary,plantaricin PlnE and PlnF were synthesised by Invitrogen.The aim genes were inserted into a bacterial expression vector pGEX-4T-1 to construct two prokaryotic expression vectors of pGEX-4T-E and pGEX-4T-F.After IPTG induction and express optimization,the fusion proteins were purified by affinity chromatography and detected by SDS-PAGE.The results showed that expression vectors were constructed correctly,more than 30% pure fusion proteins were obtained,which indicated peptides PlnE and PlnF could be accurately expressed in prokaryote cells,and lay a foundation for further researches on their bioactivity.