中文摘要：

通过两种还原型微生物铁还原菌JF-5和硫酸盐还原菌SRB对模拟酸矿废水中Fe3＋和SO2- 4的还原作用合成纳米FeS,并将该生物纳米FeS包覆在灰岩表面,以提高灰岩可渗透反应墙（PRBs）对酸矿废水中砷的去除能力。通过批吸附实验研究As（Ⅴ）的静态吸附机理,柱实验研究As（Ⅴ）在包覆灰岩柱中的动态吸附和迁移,结果表明,包覆层生物FeS粒径为纳米级,并呈现一定晶形,能有效提高灰岩表面的比表面积和对As（Ⅴ）的吸附能力,红外光谱分析表明化学吸附为主要吸附机制;生物纳米FeS包覆灰岩静态吸附实验最大吸附量为187.46μg/g,达到纯灰岩吸附量（6.64μg/g）的30倍;JF-5和SRB形成的生物包覆吸附性质优于SRB和Fe（Ⅱ）,二者对As（Ⅴ）的吸附能力都远大于纯灰岩对As（Ⅴ）的滞留能力。

英文摘要：

In this study, the experiments employed two kinds of reduced microbial, Acidiphilumcryptum JF-5 and sulfate reducing bacteria （SRB）, which can reduce Fe3＋ and SO4 2- in acid mining drainage （AMD） respectively. When reduction was done, they were mixed to form nano FeS, and then it was coated onto the particle limestone to improve the capability of removing arsenic through limestone permeable reactive barriers （PRBs）. Batch adsorption experiments were conducted to explore the static adsorption mechanism of As（ V ） and column experiments were performed for dynamic adsorption and migration of As （ V ） in FeS-eoated limestone. Some conclusions were reached： （1） The coated biological FeS was of nanometer size and assumed crystalline form, which effectively improved the surface area of the limestone and the capacity for adsorbing arsenic. Infrared ab- sorption spectrum showed that the main adsorption mechanism was chemical adsorption. （2） The maximum adsorption amount of limestone coated with bio-nano FeS, was 187.46 μg/g, which was 30 times that of the adsorption amount of pristine limestone （6.64 μg/g）. （3） The retentive capability of JF-5 and SRB coated lime- stone was better than that of the SRB and Fe（Ⅱ）, and both of them were much better than the retentive capability of pristine limestone.