中文摘要：

目的探究Th17细胞和调节性T细胞（Treg）失衡在急、慢性大鼠免疫性肝损伤模型中的意义和作用。方法将雄性Wistar大鼠随机分为3组。尾静脉注射刀豆蛋白A（ConA）分别建立急性肝损伤模型组（AC1和慢性肝损伤模型组（CC），尾静脉注射PBS作为健康对照组（HC）。流式细胞分析术（FCM）检测Th17细胞和Treg细胞频率变化，并计算Th17/Freg比值；免疫组织化学方法检测Foxp3、RORγt蛋白表达情况；ELISA法检测外周血IL-17、IL-6、IL-10、TGF—β等相关细胞因子的含量。结果与HC组比较，AC组Th17细胞频率及其相关因子（Foxp3、IL-17、IL-6等）水平显著升高（P〈0．05），Treg细胞频率及其相关因子（RORγt、IL-10等）水平亦升高（P〈0．05），但Th17／Treg比值较正常组上升（P〈0．05）；与AC组相比较，CC组Th17细胞水平显著降低，Treg细胞显著升高，Th17／Treg比值较正常组下降（P〈0．05）。结论Th17/Freg比值在急性免疫性肝损伤模型中明显上升，在慢性肝损伤模型中明显下降，Th17／Treg的失衡可能与肝损伤的发生和发展密切相关。

英文摘要：

To observe the imbalance between Th17 cells and Treg cells in acute and chronic immunological liver injury models, we randomly divided male Wister rats into three groups, and built acute and chronic models by caudal vein injection, health control group by PBS injection. The rate of Th17 and Treg was measured by the flow cytometer and then the ratio of Th17/Freg was calculate. The immunohistochemical method was applied to test the expression of Foxp3 and RORγt in tissue. And ELISA method was used to measure related cytokines such as IL-17, IL-6, IL-10, and TGF-β. Data showed compared with the health group, the Th17 and Treg rate in acute group increased （P〈0.05）, and the ThlTfFreg ratio increased remarkably. When comparing acute group, the Thl7 cells rate decreased but the Treg cells rate increased, so the ratio of Th17/Freg decreased remarkably in chronic group. All result indicated that the ratio of Th17/Treg increases in acute model and decreases in chronic model, which suggest that the imbalance between Th17/Freg may really associate with the occurrence and development of liver injury.