中文摘要：

采用对峙培养法和生长速率法,从29个木霉菌株中筛选出3株对金黄壳囊孢菌具有显著抑制效果的菌株T-33,T-14,T-09,并对3个菌株的抑菌活性成分进行复筛确定T-33菌株培养液的正丁醇浸提物对金黄壳囊孢菌菌丝生长的抑制率最高,达到94%,并能显著抑制分生孢子萌发。该抑菌活性成分可显著提高金黄壳囊孢菌细胞膜的通透性,降低病原菌蛋白质含量和SDH,PK,HK,LDH,MDH与辅酶Ⅰ的活力,同时导致Na＋,K＋-ATP酶,Mg2＋-ATP酶和Ca2＋-ATP酶活性急剧下降。结果表明：金黄壳囊孢菌细胞膜脂质过氧化严重,糖酵解途径、TCA循环、电子传递与氧化磷酸化过程等代谢途径均不能正常进行,产能下降。抑菌活性成分通过破坏病原菌的防护系统和代谢途径抑制其生长。

英文摘要：

Three Trichoderma strains, T-33,T-14,T-09, which have inhibiting effects on Cytospora chrysosperma, were derived from 29 domestic and foreign strains with methods of antagonistic culture and growth-inhibition. The extracts obviously inhibited mycelium growth and conidia germination of Cytospore chrysosperma were gained. The effect of the most highly efficient extract on pathogen’s cell physiological indicators and protective enzyme activities was systematically studied. The growth-inhibiting rate of the substances extracted from the fermented liquid of strain T-33 was 94%, and the extract also had an inhibiting effect on spore germination. The extract was able to increase the electrical conductivity and MDA content of the pathogen significantly, reduce the protein content and activity of SDH, PK, HK, LDH, MDH, and coenzyme I, and cause a sharp decline in Na＋、K＋-ATP, Mg2＋-ATP and Ca2＋-ATP enzymes activities. The results indicated that the pathogen cell membrane would be severely peroxided, and some metabolic pathways such as glycolysis, TCA cycle, electron transport and oxidative phosphorylation, would be restricted. Finally, the extract inhibited pathogen growth by destroying the pathogen’s protection system and metabolic pathways.