中文摘要：

采用组培微环境自动控制系统，研究了光合环境中光合光量子通量密度（PPFD）、CO2浓度及培养基中蔗糖和无机盐含量对驱蚊香草无菌苗生根的影响。结果表明，PPFD为100μmol·m^-2·s^-1、CO2为1400μmol·mol^-1时，无菌苗的单株鲜重、单株干重、株高增幅、根系活力及叶绿素含量显著提高，而在对照条件下即PPFD为40μmol·m^-2·s^-1、CO2为400μmol·mol^-1时，这些生理指标降低，二者差异显著。不同培养基组分对无菌苗生根影响显著，其中含20g·L^-1蔗糖的培养基优于无糖培养基，1／2MS培养基优于MS培养基。结论：PPFD为100μmol·m^-2·s^-1、CO2为1400μmol·mol^-1，含20g·L^-1蔗糖的1／2MS培养基有利于驱蚊香草无菌苗生根培养。

英文摘要：

To improve rooting of Pelargonium odoratissmum plantlets in vitro, the effects of photosynthetic environment and different components in medium were investigated. The photosynthetic photon flux density （PPFD） and concentration of CO2 as well as the sugar and salinity in medimn were controlled under the micro-environment control system. The results showed that： fresh and dry weight, increment of height, root vigor and chlorophyll content of plantlets were improved obviously with PPFD of 100μmol·m^-2·s^-1 and CO2 of 1 400μmol · mol-1 ; however, the plantlets grown under PPFD of 40 μmol · mol-2· s^-1 and CO2 of 400 μmol · mol-1 showed decreased values of these growth variables. There was significant difference between the condition of CO2 enrichment with high PPFD and the contrast. Different components in medium had significant differences on rooting of plantlets： the sugar （20 g · L^-1 ） and 1/2MS medium were better than sugar-free and MS medium respectively. We concluded that under the photosynthetic environment PPFD of 100 μmol·m^-2·s^-1, CO2 of 1 400 μmol · mol-1 , appropriate sugar （20 g · L^-1 ） and salinity （ 1/2MS medium） were beneficial to rooting of P. odoratissrnum plantlets in vitro.