中文摘要：

用酿酒酵母（Saccharyomy cescevisne）泛素融合蛋白基因从COGEME（Consortium for the functional genomics of microbial eukaryotes）植物病原菌EST（Expressed sequence tag）库中BLAST（Basic local alignment search tool）得到栗疫病菌的泛素融合蛋白EST，设计引物从栗疫病菌cDNA中克隆到该基因并测序，得到在进化上高度保守的基因，该基因开放阅读框为387bp，编码128个氨基酸残基组成的泛素融合蛋白前体；由cDNA序列推定的氨基酸序列分析结果表明，泛素融合蛋白前体包括氮末端的泛素结构域（76个氨基酸残基）和碳末端的核糖体蛋白L40结构域（52个氨基酸残基）。该蛋白为高碱性蛋白，碳末端含有一个“锌指”模式结构。与19个物种比较的结果表明，栗疫病菌与真菌泛素融合蛋白氨基酸序列相似度较高，具有高度的保守性。

英文摘要：

Using ubiquitin fusion protein gene of Saccharyomyces cerevisae as query, BLAST algorithm in the EST pool of COGEME phytopathology was performed and one highly homologous contig which contained the Cryphonectria parasitica ubiquitin fusion protein coding sequence was obtained. With the designed primers, the gene was cloned from the cDNA of C. parasitica and sequenced ,finally conserved cDNA sequence was obtained. The gene has an open reading frame of 387 bp, encoding a 128 amino acid ubiquitin fusion protein precursor composed of a 76 amino acid ubiquitin domain and followed by a 52 amino acid ribosomal protein L40 domain. The deduced precursor protein from the nucleotide sequence is highly basic. The carboxyl terminus of the ubiquitin fusion protein contains the zinc-finger motif. The predicted amino acid sequence of ubiquitin fusion protein is highly conserved when compared to the sequences of homologous proteins from twenty diverse species.