中文摘要：

目的分离、纯化和鉴定中药前胡诱导水稻抗稻瘟病的活性成分。方法以诱导水稻抗稻瘟病的活性为追踪目标,对前胡的丙酮提取物进行硅胶柱层析分离得到活性最强的部分,再用SephadexLH-20和液相制备色谱分离得到诱抗活性的主活性成分,采用紫外-可见光谱、红外光谱、ESI-MS质谱和核磁共振（1HNMR和13CNMR）波谱法对其进行结构解析与鉴定。结果该植物的丙酮提取物通过硅胶柱层析得到7个馏分,其中4、5、6三个馏分对水稻稻瘟病有较强的诱导抗病性,质量浓度为50mg·L-1时,其诱抗效果分别为55.18%、59.54%、54.36%,经过SephadexLH-20和液相制备色谱分离得到3个主活性成分,质量浓度为10mg·L-1时,其诱抗效果分别为52.77%、60.76%、60.28%,波谱结构解析表明,三者分别为北美芹素、前胡香豆素Ⅱ、前胡香豆素Ⅲ,其中前胡香豆素Ⅲ为国内首次从前胡中分离得到。结论香豆素是前胡诱导水稻抗稻瘟病的主要活性成分。

英文摘要：

Objective The active components of Radix peucedani capable of inducing rice resistance to blast disease were separated, purified and identified.MethodUsing activities of inducing resistance to blast disease as an activity monitoring parameter, acetone extract was isolated by silica-gel column to obtain the strongest part of the activity, and the main resistance inducing components from the strongest fractions were separated by using Sephadex LH-20 resin and the structures were analyzed by ultraviolet-vis, infra-red, ESI-MS, 1H-NMR and 13C-NMR spectrums.ResultSeven fractions from A1 to A7 collected from silica-gel column were tested, A4, A5, A6 had the strongest resistance inducing activities.When the concentrations were 50 mg·L-1, the resistance inducing activities were 55.18%, 59.54%, and 54.36%, respectively. Then three active compounds, Q-1, Q-2, and Q-3, were separated from A4, A5, and A6 through Sephadex LH-20 resin and HPLC. When the concentrations were 10 mg·L-1, the resistance inducing activities of Q-1, Q-2, and Q-3 were 52.77%, 60.76%, and 60.28%, respectively. The spectroscopy analysis indicated that the 3 active components of inducing rice resistance to blast disease of Radix peucedani were peucedanocoumarin Ⅲ, peucedanocoumarin Ⅱ, pteryxin. In addition, peucedanocoumarin Ⅲ was isolated from Radix Peucedani for the first time in China. Conclusion It is concluded that the coumarin compounds are the most important substance of Radix peucedani capable of inducing rice resistance to blast disease.