中文摘要：

根据不同领域的需要，控制蛋白质在材料表面的吸附是一个具有重要应用价值的课题。聚（N-异丙基丙烯酰胺）（PNIPAAm）改性表面能够响应外界温度变化从而改变其表面性质，这一特点为调控蛋白质的吸附提供了可能。近年来，研究者们应用多种表征方法考察了不同温度下蛋白质在PNIPAAm改性表面的吸附，并试图从分子水平上深入理解其吸附机制及影响因素。本文综述了近年来应用PNIPAAm改性表面对蛋白质吸附的研究及其最新进展。发现当PNIPAAm层厚度处于一定范围内时，PNIPAAm改性表面表现出对蛋白质吸附的温度敏感性，并可以利用这一性质将其应用于蛋白质纯化及分离和生物传感器等领域。而当PNIPAAm层厚度超过一个临界值时，PNIPAAm改性表面表现出良好的阻抗血浆蛋白质的性质，使其有望在血液相容性表面领域得到应用。最后，就PNIPAAm改性表面调控蛋白质吸附的未来发展方向简要地进行了展望。

英文摘要：

Control over the adsorption of proteins on surfaces according to the specific requirements of different fields is of crucial importance for various applications including implanted devices, biosensors, tissue engineering, and separation sciences. Surfaces modified by poly （N-isopropylacrylamide） （PNIPAAm） are capable of reversibly altering their properties in response to the change of environmental temperature, providing possibility to regulate protein adsorption on surfaces. In recent decades, considerable attentions have been paid to investigating protein adsorption on PNIPAAm modified surfaces under different temperatures using various characterization methods, and trying to understand the mechanism and influence factors in molecular level. In this paper, the recent progress on studies of protein adsorption on PNIPAAm modified surfaces are reviewed. It is found that when the thickness of PNIPAAm layer is within a certain range, PNIPAAm modified surfaces exhibit thermo-responsivity of protein adsorption, which can be used in applications of protein purification and separation as well as biosensors. On the other hand, when the thickness of PNIPAAm layer is beyond a critical thickness, PNIPAAm modified surfaces show good resistance to plasma proteins, making them beneficial for applications as hemocompatible surfaces. In the end, the directions of future development on regulation ofprotein adsorption using PNIPAAm modified surfaces are proposed.