以头肾组织为材料,采用植物血球凝集素(phytohemagglutinin,PHA)、秋水仙素溶液腹腔注射和空气干燥制片法,对海南野生三斑石斑鱼(Epinephelus trimaculatus)的染色体组型进行研究,结果表明,海南野生三斑石斑鱼具有48条染色体,其核型公式为2n=48,48t,臂数NF=48。未发现与性别相关的异型染色体。基于目前已研究过的23种石斑鱼核型进行统计,推测2n=48,48t,NF=48可能为石斑鱼属的基本原始核型。
In our study,the head kidney tissue were used as materials,and in vivo treatment by phytohemagglutinin(PHA) and colchicines and Giemsa staining were performed,and the cytogenetics of E.trimaculatus were analyzed.The results indicated that the karyotypic formula of E.trimaculatus was 2n=48,48t,NF=48,no heteromorphic chromosome was detected.Based on the karyotypes of 23 species of groupers,the conclusion that 2n=48,48t,NF=48 was the primitive cytogenetic characterization of genus Epinephelus were proposed.