中文摘要：

目的：研究自养、异养两种营养模式下斜生栅藻（Scenedesmus obliquus）生物量和生化组分含量变化。方法：称重法、有机溶剂抽提法、苯酚-硫酸法、Lowry法和分光光度法测定生化组成含量。结果：自养培养时最大生物量为6.95g/L,异养培养时的最适碳、氮源分别为葡萄糖和酵母提取物,以不同浓度葡萄糖和酵母提取物培养时所获得的最大生物量分别为7.94g/L和5.39g/L。自养有利于脂质积累,异养培养有利于碳水化合物和蛋白质的积累。自养时低光、高氮浓度条件有利于色素的积累,异养时色素积累随着葡萄糖浓度的升高而降低,并随酵母提取物浓度的升高而升高。结论：不同营养模式下斜生栅藻的生长及生化组分存在差异,在营养模式改变时其代谢方式发生了变化。

英文摘要：

Objective： The changes of growth and biochemical components in Scenedesmus obliquus cultured in autotrophic and heterotrophic conditions were investigated. Method： The gravimetric method,organic solvent extraction,phenol- sulfuric acid,Lowry,s method and spectrophotometry were used to measure the biomass concentration and biochemical components in those two trophic modes. Result： The highest biomass concentration was up to 6. 95 g / L under photoautotrophic condition. The optimal organic carbon and nitrogen sources were glucose and yeast extract under heterotrophic culture. The maximum biomass concentrations were reached to 7. 94 g / L and 5. 39 g / L under the different concentrations of glucose or yeast extract,respectively. High content of lipids could be accumulated in S. obliquus under photoautotrophic condition,while massive carbohydrate and protein were produced in heterotrophic condition. Under photoautotrophic culture,the lower light and higher nitrogen concentration were conducive to accumulate pigment. And under heterotrophic culture,the pigment content was increased following the glucose concentration decreased and yeast extract concentration increased. Conclusion： The growth and biochemical components content had a certain difference in various trophic modes. The results showed that the changes in metabolic pathway were resulted from alteration of nutritional conditions.