通过构建填料型微生物燃料电池(MFC),首次对以喹啉为燃料时的MFC阳极表面的微生物群落进行了分析.PCR-DGGE的试验结果表明,随着燃料的改变,微生物群落也发生改变.当以喹啉和葡萄糖的混合溶液稳定地作为燃料时,由于受到喹啉毒性的抑制,微生物多样性降低,优势菌也发生明显的改变.与葡萄糖共基质相比,以单一喹啉为燃料时的阳极微生物优势菌落发生明显改变.新增加一类菌,这类菌与Pseudomonas sp.DIC5RS的同源性为100%,推测该菌在单一喹啉为MFC燃料时喹啉的降解过程中起到关键作用.
A graphite-packed MFC was constructed to investigate anode microbial community firstly in the MFC when using quinoline as fuel.PCR-DGGE analysis showed that microbial community changed with the substrates.Due to the inhibition of quinoline toxic ability,microbial species diversity decreased and the dominating species changed significantly when using quinoline-glucose mixture.Compared to the mixed substrates with glucose,the microbial community changed significantly when the substrate changed to sole quinoline.Furthermore,a new species was found,which had the highest homology(100%) with Pseudomonas sp.DIC5RS.We speculated that this strain played the key role in degradating quinoline when using sole quinoline as the fuel.