中文摘要：

膨胀床吸附技术可以直接从细胞破碎液（未澄清液）中提取目标产物。而无需预先进行固液分离操作，具有快速、高效和集成化的特点。在实际应用中，料液中细胞（碎片）的存在将会影响到膨胀床的床层稳定性和操作性能。Lin等发现在不考虑细胞与介质相互作用条件下料液切换对膨胀床的稳定性几乎无影响。

英文摘要：

Upon the application of 3 % yeast suspension as a feedstock to an expanded bed of Streamline AC equilibrated with 0.02 mol · L^-1 phosphate buffer （pH 7.0）, the transient variations of local bed voidage and particle size distribution were investigated. The experiments were carried out in a customized glass column （26 mm I.D. ） modified by side ports. It was exhibited that the introduction of unclarified feedstock with a high viscosity and density induced the upward movement of particle and consequently a change in local bed voidage and particle size distribution. At a given bed height, voidage firstly decreased to a minimum and then increased to a high equilibrated value in voidage. The transient process not only included a fluctuation in particle size distribution but also involved the change in particle concentration at a certain bed height, particularly at the middle and top of the column.