中文摘要：

目的 探讨小鼠胚胎胃肠道和胎盘组织的菌群构成.方法 采集昆明系孕鼠的胚胎组织和胎盘组织24份,分别为孕早期（孕12- 13 d）胚胎胃肠道组织、孕晚期（孕19 -20 d）胚胎胃肠道组织和孕晚期（孕19 -20 d）胎盘组织各8份.应用血液或组织DNA提取试剂盒提取胃肠道组织和胎盘组织菌群的DNA,然后将24个样本所提取的DNA进行高通量测序.统计学方法采用方差分析.结果 孕鼠胚胎胃肠道组织及胎盘组织的菌群在“门”水平有4个优势菌群,分别是变形菌门（95.00％、88.14％、87.26％）,拟杆菌门（1.71％、2.15％、2.63％）,放线菌门（1.16％、4.10％、3.38％）及厚壁菌门（0.75％、2.62％、2.01％）.在科水平有9个优势菌科,其中以肠杆菌科（46.99％、44.34％、41.08％）、希瓦菌科（21.99％、21.10％、19.05％）、莫拉菌科（9.18％、7.09％、5.64％）为主.菌群种类孕早期和孕晚期胚胎胃肠道菌群分别为65.44％和62.73％,与孕晚期胎盘组织菌群相同.菌群丰度“科”水平3组相同菌群的含量分别占78.16％、72.53％及65.78％.结论 胚胎胃肠道和胎盘组织有菌群定植,同时对其进行了分类.

英文摘要：

Objective To explore the composition of the gastrointestinal bacterial flora of mouse embryos and the placenta tissue bacterial flora.Method Twenty-four specimens were collected from pregnant Kunming mouse including 8 mice of early embryonic （12-13 days） gastrointestinal tissues,8 cases of late embryonic （19-20 days） gastrointestinal tissues,8 of late pregnancy placental tissues.The 24 samples were extracted by DNeasy Blood ＆ Tissue kit for high-throughput DNA sequencing.Result The level of Proteobacteria,Bacteroidetes,Actino-bacteria and Firmicutes were predominantin all specimens.The relative content of predominant bacterial phyla in each group：Proteobacteria （95.00%,88.14%,87.26%）,Bacteroidetes （1.71%,2.15%,2.63%）,Actino-Bacteria （1.16%,4.10%,3.38%）,Firmicutes（0.75%,2.62%,2.01%）.At the level of family,there were nine predominant bacterial families in which Enterobacteriaeae,Shewanel laceae and Moraxellaceae were dominant.The relative content of dominant bacterial family in eachgroup：Enterobacteriaeae （46.99%,44.34%,41.08%）,Shewanellaceae （21.99%,21.10%,19.05%）,Moraxellaceae（9.18%,7.09%,5.64%）.From the species of flora,the flora from fetal gastrointestinal in early pregnancy and late pregnancy （65.44% and 62.73%） were the same as that from placenta tissue in the late pregnancy.From the abundance of bacteria,at the level of family,the same content of bacteria in three groups accounted for 78.16%,72.53% and 65.78% respectively.Conclusion It was proved that the gastrointestinal bacterial flora of mouse embryos and the placenta tissue bacterial flora were colonized.At the same time the bacteria are classified.