中文摘要：

目的评估单纯疱疹病毒腺苷激酶(herpes simplex virus-thymidine kinase,HSV-tk)基因对髓母细胞瘤细胞系Daoy的杀伤作用。方法 HSV-tk基因稳定转染人的髓母细胞瘤细胞系Daoy,获得Daoy-tk细胞;Daoy-tk细胞与Daoy细胞按不同比例混合,给予不同浓度的更昔洛韦(ganciclovir,GCV),7天后应用噻唑蓝(MTT)法测定细胞生存率。结果 Daoy-tk在GCV浓度为3μg/ml时,即可对Daoy细胞产生最大杀伤作用;Daoy-tk与Daoy细胞比例达到1:4时Daoy-tk仍有明显的抗肿瘤作用。结论 HSV-tk/GCV系统对人的髓母细胞有明显的杀伤作用。

英文摘要：

Objective To evaluate the antitumor effect of herpes simplex virus-thymidine kinase gene(HSV-tk ) on medulloblastoma cell line Daoy.Methods HSV-tk gene was stably transfected into Daoy cells to gain Daoy-tk cells.Daoy-tk and Daoy cells were cocultured at various ratios and treated with different concentrations of ganciclovir(GCV). The viability of the cells was measured with MTT assay at day 7 after coculture.Results Daoy-tk cells have the strongest killing effect on Daoy cell line medulloblastoma while GCV...