中文摘要：

为建立条纹锯鮨精液超低温冷冻保存方法,实验采用计算机辅助精子分析系统（CASA）分析了采用6种抗冻保护剂（GLY[甘油]、DMSO[二甲基亚砜]、PG[丙二醇]、EG[乙二醇]、METH[甲醇]、DMA[二甲基乙酰胺]）在4种浓度（5%、10%、15%、20%,v/v）下对条纹锯鮨精液的冷冻保存效果。结果发现,以HBSS为稀释液,采用程序降温仪分步降温冷冻保存条纹锯鮨精液,37℃水浴解冻后的精子中,15%PG作为抗冻保护剂的精子运动率最高,达到（93.1±0.9）%,与鲜精差异不显著（P〉0.05）,15%PG作为抗冻保护剂的精子水浴解冻后精子的运动速度最高,平均直线速度、平均曲线速度、平均路径速度分别达到了（88.3±0.3）μm/s、（76.2±0.5）μm/s、（86.7±0.7）μm/s,与鲜精差异不显著（P〉0.05）。在不同种类及不同浓度抗冻保护剂保护下,15%PG作为抗冻保护剂的精子解冻后1 min内运动率变化与鲜精差异不显著（P〉0.05）。研究表明,15%PG为条纹锯鮨最佳抗冻保护剂,可用于条纹锯鮨精液的超低温冷冻保存。

英文摘要：

In order to establish Centropristis striata sperm cryopreservation method, this paper used a computer- assisted sperm motion analysis system（CASA）, and selected six cryoprotectants, such as glycerin （GLY） , dimethyl sulfoxide （ DMSO ） , propylene glycol （PG） （DMA）, methanol（METH） at four concentrations （5% , 10% ethylene glycol （ EG）, dimethyl sulfoxide 15% and 20% , V/V） in HBSS to find the influence of C. striata sperm cryopreservation. Results showed that the best cryopreservation protocol for C. striata was 15% PG as antifreeze protective agent in HBSS with step cooling scheme. After 37℃ water bath thawed, motility of the sperm reached （93.1 ± 0.9） % , which was higher than other combination groups and showed no significant difference with fresh sperm （P 〉 0.05 ）. The group of 15 % PG performed best in average linear velocity, average velocity, and average path velocity at （88.3 ±0, 3） μm/s, （76.2 ±0.5 ） μm/s, and （86.7 ± 0.7） μm/s, which showed no significant difference with fresh sperm （P 〉 0.05）. In different cryoprotectants with different concentration, the motility of 15% PG within 1 min had no significant difference with fresh semen. Therefore, 15% PG was the best cryoprotectant for C. striata and could be applied in practice.