中文摘要：

将包含Pib基因启动区及下游完整编码区的9.9kbDNA片段克隆到双元载体pPZP2Ha3（＋）中,构建了35S驱动的正义表达载体pNAR701（20.3kb）;同时将Pib基因编码区6986～9392bp之间的DNA片段,克隆到双元载体pPZP2Ha3（-）中,构建了35S驱动的反义表达载体pNAR703（12.8kb）;用农杆菌介导法转入中感稻瘟病水稻品种R109中。PCR、Southern blot鉴定以及转基因T0代种子的潮霉素抗性鉴定证明,目的基因已经整合到R109基因组中,并能在后代稳定遗传。Northern blot分析表明含有启动区及下游完整编码的Pib基因片段在35S驱动下能够在转基因后代中表达。对T1代苗期转基因植株和分蘖期离体叶片进行抗稻瘟病初步分析,结果显示pNAR701转基因植株对稻瘟病生理小种ZD1和ZG1的抗性较对照增强,而转反义片段的pNAR703转基因植株对稻瘟病的抗性较对照减弱。

英文摘要：

The promoter region and intact coding region of Pib gene （9.9 kb） was inserted into the downstream of CaMV 35S promoter in a binary vector pPZP2Ha3（＋）, resulting a plasmid pNAR701. And a fragment of Pib gene from 6 986 to 9 392 bp was placed into pPZP2Ha3（-） under the control of CaMV 35S promoter, producing an antisense expression vector pNAR703. These two recombined vectors were transferred into a blast medium susceptible rice cultivar R109 by an Agrobecterium-mediated method. Tests of PCR and Southern blotting for transgenic plants as well as the segregation of hygromycin resistance in T1 generation confirmed that the target DNA fragments were integrated into genome of R109 and hereditable. Northern blotting analysis showed the coding region of Pib gene double driven by 35S and its native promoter was able to transcript in T1 transgenic plants. Rice blast resistance test for T1 transgenic seedlings of 3-4 leaves stage and in vitro leaves in filleting stage showed that transgenic plants of pNAR701 were more resistant to blast race ZD1 and ZG1 than the wild type plants, but the resistance of antisense transgenic plants from pNAR703 was decreased compared to the controis.