中文摘要：

为了对猪Toll样受体（TLR）3、7和8基因进行克隆与序列分析,本实验从猪肺泡巨噬细胞（PAM）中,利用RT-PCR方法分片段扩增猪TLR3、7、8基因,并克隆于pMD18-T载体中。根据测序结果分析,猪TLR3、7和8基因的ORF分别为2718bp、3153bp和3087bp,并分别编码906、1051和1029个氨基酸。同源性分析结果显示,它们与GenBank中登录的猪TLR的相应序列同源性达99%以上;与牛、马、羊和人的同源性较高,与鼠的同源性次之,与鸡的同源性最低,其蛋白分子结构预测表明猪TLR3、7、8均为跨膜蛋白。

英文摘要：

Toll-like receptors （TLR） were the key component of innate immune system. In the present study,porcine Toll-like receptor 3,7 and 8 （pTLR3,pTLR 7 and pTLR 8） genes were amplified by RT-PCR from porcine alveolar macrophages （PAM）. The PCR products were cloned and sequenced. The results showed that the pTLR3,pTLR7 and pTLR8 genes contained ORF of 2,718 bp,3,153 bp,3,087 bp in length that encoded 906 amino acids （aa）,1,051 aa and 1,029 aa,respectively. The nucleotide sequences of pTLR3,pTLR7 and pTLR8 shared 99% homology with the published pTLR sequences in GenBank. The structure prediction showed that the pTLR3,pTLR7 and pTLR8 were transmembrane proteins.