欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
以磁性壳聚糖微球为载体，采用交联法制备磁性固定化纤维素酶．分别研究了活化剂乙基[3-（二甲胺基）丙基]碳二亚胺盐酸盐（EDC）与戊二醛的活化时间及活化温度对酶固载量的影响．比较磁性固定化纤维素酶与游离纤维素酶在外加磁场作用下，其酶活力的变化．结果表明，磁性固定化纤维素酶的酶活受磁场作用影响较小；当磁场强度＜500mT与曝磁时间＜60min时，游离纤维素酶的酶活随着磁场强度与曝磁时间的增加而增加，但超过这范围，酶活就急速下降．
英文摘要：
Magnetic immobilized enzymes are potential in bioteclmological industry because of their rapid separation and easiness to control. Magnetic immobilized cellulases were prepared using the magnetic chitosan micresperes as carriers and by the croaslinkage method. The effects of activation time and activation temperature of the activators of 1-Ethyl-3-（3-dimethylaminopropyl） -carbodiimide hydrochloide（EDC） and glutaraldehyde on the protein binding capacity were investigated. The activities of the magnetic immobilized cellulases and free cellulases exposed to the external magnetic field were measured. It was indicated that the activities of the magnetic immobilized cellulases did not change under the external magnetic field, while the activities of free cellulases increased with the increase of the intensity of the magnetic field and the exposure time when the magnetic intensity was less than 500 mT and the expesure time was less than 60 min, however, out of this range the activities decreased dramatically.
同期刊论文项目
同项目期刊论文
Study of Starch Gelatinization in a Flow Field using Simultaneous Rheometric Data Collection and Mic
Comparative Study of Inactivation and Conformational Change of Lysozyme Induced by Pulsed Electric F
Purification and Characterization of a Novel Fibrinolytic Enzyme from Bacillus sp. Nov. SK006 Isolat
Effect of high pressure treatment on some physicochemical and functional properties of soy protein i
Specific and rapid detection of foodborne Salmonella by loop-mediated isothermal amplification metho
Analysis of genetic determinants involved in multi-resistance in clinical strains isolated from rena
Dehydration of concentrated Ganoderma lucidum extraction by combined microwave-vacuum and convention
Unnoticed Spread of Class 1 Integrons in Gram-positive Clinical Strains Isolated in Guangzhou, China
New array of aacA4-catB3-dfrA1 gene cassettes and a none-ORF cassette from a class 1 integron-positi
Study of thermal properties and heat-induced denaturation and aggregation of soy proteins by modulat
Purification and Partial Characterization of Lactobacillus Species SK007 Lactate Dehydrogenase (LDH)
Effect of thermal pretreatment of raw soymilk on the gel strength and microstructure of tofu induced
Acetylated Starch-based Biodegradable Materials with Potential Biomedical Applications as Drug Deliv
Distribution and characterization of integrons in various serogroups of Vibrio cholerae strains isol
Influence of transglutaminase-induced cross-linking on in vitro digestibility of soy protein isolate
Purification and characterization of L-arabinose isomerase from Lactobacillus plantarum producing D-
Usefulness of Sau-PCR for molecular epidemiology of nosocomial outbreaks due to Burkholderia cepacia
Effect of cross-linkage of POCI3 on invitrodigestibility and formation of enzyme resistant starch of
Loop-mediated isothermal amplification method for rapid detection of the toxic dinofagellate Alexand
Effect of processing parameters on the properties of transglutaminase-treated soy protein isolate fi
Nosocomial infection caused by class 1 integron-carrying Staphylococcus aureus in a hospital in Sout
Effect of high-intensity pulsed electric fields on the activity, conformation and self-aggregation o
期刊信息
位置：