中文摘要：

针对现有的胆红素检测方法大都需要特殊的仪器以及受某些适用范围的影响而缺乏选择性等问题,提出基于金纳米簇的胆红素荧光检测方法。以牛血清白蛋白（BSA）为模板在生理条件下制备荧光金纳米簇,BSA起到稳定剂和还原剂的作用,将氯金酸的金离子还原,形成稳定的金纳米簇。由于牛血清白蛋白能与游离胆红素形成复合物,当BSA保护的金纳米簇与胆红素作用时,胆红素会使金纳米簇的荧光发生猝灭现象。基于游离胆红素与BSA作用使金纳米簇的荧光猝灭的原理,建立了以金纳米簇为荧光探针的胆红素检测方法。实验结果表明,反应时间为8h,反应碱度为pH=13,检测缓冲溶液的pH值为7.5时,胆红素检测灵敏度最高,标准曲线方程为：F0/F-1=0.0971C-1.012,R=0.998,线性范围为11.19~23.14μmol/L。人体血液中的常见成分如果糖、半乳糖、多巴胺、葡萄糖等不会干扰胆红素的测定,因此该检测方法具有很强的特异性,可实现对血清中胆红素的高灵敏度和高选择性检测。

英文摘要：

Gold nanoclusters（AuNCs） were prepared by using bovine serum albumin（BSA） as a template under physiological conditions, where BSA acted as a reducing agent and stabilizer. Since BSA can form complexes with the free bilirubin, the red fluorescence of AuNCs were quenched when bilirubin was exposed to BSA protected AuNCs. The fluorescence quenching degree increased with the increment of bilirubin concentration. In order to obtain high-sensitivity detection of bilirubin by u- sing BSA-gold nanoclusters, the experimental conditions for the synthesis of AuNCs such as reaction time, pH were opti- mized. And the effect of buffer pH of bilirubin detection was also investigated. Experimental results show that the highest detection sensitivity is obtained when the reaction time is 8 h, the reaction alkalinity pH is 13, buffer solution pH is 7.5. The calibration curve can be expressed as F0/F-1=0. 097 1C-1. 012 in the concentration range of 11.19-23.14 μmol/L. Interference experiments were also studied to examine the effects of some common ingredients in human blood on bilirubin measurements. The results showed that fructose, galactose, dopamine, glucose does not interfere with the determination of bilirubin.