中文摘要：

目的检测35例ALL患儿诱导缓解治疗过程中血浆左旋门冬酰胺酶（L—Asp）活性以及抗L—Asp抗体水平变化，并分析它们之间的相关性。方法通过光度滴定法检测血浆L—Asp活性水平变化；通过ELISA方法检测血浆中抗L-Asp抗体永平变化。结果35例ALL患儿在诱导缓解治疗过程中，L-Asp活性始终保持在较高水平，中位活性为539U／L，无1例酶活性水平在100U／L以下；在L-Asp使用期间及停用后7天时，血浆中抗L-Asp抗体水平无明显变化，只是在停用第十天时抗体水平才有所升高，但与对L-Asp产生过敏患儿的抗体水平相比还有约4倍差距。结论ALL患儿诱导缓解治疗过程中使用L-Asp时血浆中抗L-Asp抗体的产生对L—Asp的作用无显著影响。

英文摘要：

Objective To estimate the presence of anti - asparaginase antibodies and to correlate the results with L-asparaginase enzymatic activity during the treatment. Methods 35 children with newly diagnosed acutte lymphoblastic leukemia （ALL） were investigated. All patients were treated according to the ALL-XH-99 protocol. L-asparaginase activity was estimated in serum samples, collected before each L-asparaginase administration, using the photometrical method. Anti-asparaginasc antibody concentration was measured by enzyme -linked immunosorbent assay （ELISA） at the four time points： 1 - 2 h before the second dose, the last day of L-asparaginase treatment in the induction, the seventh and tenth day after L-Asp ceased. Results The mean L-asparaginase activity was 539 IU/L in the induction phase; a slight elevated anti-asparaginase antibodies were found in this phase and kept cautiously till the tenth day after L-asp ceased, but it was four- fold lower compared with those with allergic reaction to L-asp. No allergic reactions against L-asparaginase were noted during induction. Conclusion The presence of anti -asparaginase antibodies has no significant effect to L-asparaginase activity during the induction of childhood ALL.