中文摘要：

谷胱甘肽过氧化物酶在植物响应盐胁迫中具有重要作用。依据盐芥EST序列进行RACE实验，获得1个新的谷胱甘肽过氧化物酶基因，命名为ThGPX6（GenBank注册号为FJ357244）。该基因的cDNA全长892bp，包含1个长为702bp的开放读码框．编码234个氨基酸。生物信息学分析表明，该蛋白具有植物GPX的典型结构，即GPX催化活性区（NVASKCGLT）和标志性基序（ILAFPCNQF），以及PHGPX特有序列（KwNF（S／T）KFL）。实时荧光定量PCR分析结果表明，ThGPX6在盐芥叶片和根中表达，其表达受NaCl诱导，显示ThGPX6在植物高盐响应中发挥作用。亚细胞定位结果表明，ThGPX6存在于线粒体和内体中的可能性最大，预示着ThGPX6在清除ROS过程中起着重要作用。

英文摘要：

Glutathione nalysis on EST sequence of oxidase was discovered and cleotides,including a single peroxidase（GPX） plays an important role in response to salt stress in plant. Through the a- Thellungiella halophila, a novel gene exhibiting high homology with plant glutathione per- named as ThGPX6（GenBank accession number：F J357244）. ThGPX6 consists of 892 nu- 702bp opening reading frame which encodes a 234-amino acid peptide. Bioinformatics a- nalysis showed that ThGPX6 had typical structures of GPX, such as catalysis region （NVASKCGLT）, characteristic mo- tif（ILAFPCNQF） and PHGPX special sequence （KWNF （S/T）KFL）. Quantitative real-time PCR analysis revealed that the gene expressed in shoot and root, and could be induced by NaC1 in T. halophila. These results showed that the ThGPX6 played a crucial role under salt stress in T. halophila. ThGPX6 is most likely localized in the mitochondria and endosome, suggesting importence of ThGPX6 in the process of cleaning ROS.