中文摘要：

目的 探讨精神应激大鼠的心律失常发生与胸1～5脊髓神经组织及心脏自主神经重构和心肌电重构的相关性.方法 30只SD雄性大鼠,体重180～220 g,随机分为对照组、应激组、应激＋盐酸氟西汀组（简称氟西汀组）各10只.用Cronli方法建立后两组大鼠精神应激模型.观察各组：（1）心电图变化、心律失常发生;（2）胸1～5脊髓和心肌组织的场电位时程（FAPD）和场电位时程离散度（FAPDd）变化;（3）心肌组织免疫组化、电镜和激光共聚焦下的生长相关蛋白43（ GAP-43）、乙酰胆碱酯酶（CHAT）、络氨酸羟化酶（TH）变化.结果 （1）应激组与对照组相比,体重、摄食量、水平运动、垂直运动得分、清洁动作次数等显著下降,粪便粒数显著增加.心电图出现P波、P-R间期、QRS间期及Q-T间期延长,并出现心率减慢、室性早搏（发生率为80％）.胸1～5脊髓神经FAPD显著延长[（ 144.25±12.63） ms比（79.56±8.01） ms],FAPDd显著增加[（13.3±9.11） ms比（9.36±7.01 ）ms];左右心房及左右心室肌FAPD延长[左房（122.43±19.34） ms比（92.59±7.61）ms,右房（149.89±14.68） ms比（105.18±15.94）ms;左室（162.62±7.04） ms比（110.45±6.92）ms,右室（ 152.21±30.49） ms比（131.06±12.04）ms],左右心房及左右心室肌的FAPDd也显著增加;心肌细胞排列紊乱、间质胶原纤维增生、中性粒细胞和淋巴细胞浸润;GAP-43、TH、CHAT心肌分布显著增加.（2）氟西汀组较应激组大鼠体重和摄食量增加,室性心律失常降低到20％,心电图各波段时间缩短.氟西汀组较应激组胸1～5脊髓神经的FAPD缩短[（134.32±11.23）ms比（144.25±12.63）ms],FAPDd降低[（12.74±10.11）ms比（13.3±9.11 ）ms];左右心房及左右心室肌FAPD缩短[左房（ 100.25±6.22）ms比（122.43±19.34）ms,右房（132.56±26.45） ms比（149.89±14.68） ms;左室（158.20±18.33）ms比（162.62±7.04）ms,右室（142.48±16.65）ms比?

英文摘要：

Objective The study aimed to investigate the relationship between arrhythmia occurrence and nerve remodeling of thoracic spinalcord 1-5 nerves as well as myocardial electrophysiological remodeling in a metal stress rat model.Methods Thirty SD rats （weight 180-250 g）were randomly divided into control group （n = 10）,stress group （n = 10） and fluoxetine group （n = 10,10 mg/kg i.p.for 3 weeks）.Stress model （given by unpredicted chronic mild stress） was established according to Cronli＇s protocol.Following parameters were observed：（ 1 ） ECG waveform change and arrhythmias ; （2）tissue field action potential duration （FAPD） of thoracic spinal cord 1 - 5 and cardiac tissue mapped by microelectrode arrays （ MEA ） technique; （ 3 ） myocardial growth-associated protein （ GAP-43 ）,tyrosine hydroxylase （TH）,choline acetyltransferase （CHAT） distribution observed by immunofluorescence and confocal laser scanning microscope （LSCM）.ResultsThree weeks later：（1） The body weight,food intake,consumption of sugar water,the horizontal and vertical movement score,cleaning action of rats were significantly decreased,and fecal grains significantly increased,P-wave,P-R interval,QRS-wave and Q-T interval were significantly prolonged and heart rate was significantly reduced in stress group compared with control group （ all P 〈0.05）.Incidence of ventricular premature beat was 80% in stress group and 0% in control group （P 〈 0.05 ）.The FA PD of thoracic spinal cord 1 -5 nerves [ （ 144.25 ± 12.63 ）ms vs （79.56 ±8.01 ）ms ] and of cardiac tissue [ LA （ 122.43 ± 19.34）ms vs （92.59 ± 7.61 ）ms,RA （ 149.89 ± 14.68 ）ms vs （105.18 ± 15.94） ms,LV（162.62 ± 7.04） ms vs （110.45 ±6.92） ms,RV （152.21 ± 30.49） ms vs （ 131.06 ± 12.04） ms ] were significantly prolonged,FAPD dispersion （FAPDd） significantly increased [ thoracic spinal cord 1 - 5 （ 13.3 ± 9.11 ） ms vs （ 9.36 ± 7.01 ） ms ] in stress group compared with the con