中文摘要：

根据拟南芥乙烯响应因子AtERF104的序列设计保守引物,从甘蓝型油菜品种中双9号中克隆到同源基因BnERF104。序列分析表明BnERF104蛋白含有一个典型的ERF domain保守结构域,属于乙烯响应转录因子ERF家族的成员。甘蓝型油菜用核盘菌（Sclerotinia sclerotiorum）接种6h后,BnERF104基因被诱导显著上调表达。BnERF104基因在转基因拟南芥中的超表达提高了病原相关蛋白（PR）防御素PDF1.2和几丁质酶ChiB基因的表达量,显著增强了对腐生营养型真菌核盘菌的抗性。

英文摘要：

BnERF104 gene was cloned from Brassica napus cv Zhongshuang 9 using conserved primers designed from AtERF104 gene sequence of Arabidopsis.NCBI-BLAST showed BnERF104 protein had a typical ERF conserved domain,was a member of ETHYLENE-RESPONSE-FACTOR（ERF） family.BnERF104 expression was up-regulated significantly at 6h after S.sclerotiorum inoculation.Over-expression of BnERF104 in transgenic Arabidopsis thaliana activated expressions of pathogenesis-related（PR） gene PDF1.2 and ChiB,and significantly enhanced plant resistance to necrotrophic fungi S.sclerotiorum.