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内皮-单核细胞激活多肽酶Ⅱ增强血肿瘤屏障通透性的机制

ISSN号：1006-2947
期刊名称：解剖科学进展
时间：0
页码：33-36
分类：R743[医药卫生—神经病学与精神病学;医药卫生—临床医学]
作者机构：[1]中国医科大学附属盛京医院神经外科,辽宁沈阳110004, [2]中国医科大学基础医学院神经生物学教研室,辽宁沈阳110001
相关基金：国家自然科学基金资助项目（No.81172197,81171131,30973079）; 高等学校博士学科点专项科研基金（No.20092104110015,20102104110009）; 沈阳市科学技术计划项目（No.F10-205-1-37,F10-205-1-22）
相关项目：Caveolin-1对血肿瘤屏障紧密连接调节机制的研究

作者：李振|刘云会|薛一雪|刘立波|LI Zhen1,LIU Yun-hui1,XUE Yi-xue2,LIU Li-bo2(1.Dep|2.Department of Neurobiology,College of Basic Medi|

关键词：内皮-单核细胞激活多肽酶II, 血肿瘤屏障, 通透性, 紧密连接, α-ATP合成酶, 大鼠, endothelial monocyte-activating polypeptide-II, blood-tumor barrier, permeability, tight junction, α-ATP synthase, rat

中文摘要：

目的研究内皮-单核细胞激活多肽II（endothelial monocyte activating polypeptide-II,EMAP-II）选择性开放血肿瘤屏障（blood-tumor barrier,BTB）的作用机制。方法荷瘤大鼠被随机分成4组（每组12只）：对照组,EMAP-II组,寡霉素组和寡霉素＋EMAP-II组。采用伊文思蓝（Evans blue,EB）渗透性实验评估各组BTB通透性变化情况;Western Blot法检测脑微血管内皮细胞上紧密连接相关蛋白ZO-1的表达水平变化;双重免疫荧光法检测EMAP-II和α-ATP合成酶在脑微血管内皮细胞上的分布。结果 EMAP-II组BTB通透性显著高于对照组和寡霉素组（〈0.01）,紧密连接相关蛋白ZO-1的表达水平显著降低（〈0.01）,其作用受到ATP合成酶抑制剂寡霉素（Oligomycin）的显著抑制（〈0.05）;EMAP-II与α-ATP合成酶在胶质瘤微血管内皮细胞上共定位。结论 EMAP-II可能通过开放紧密连接增强BTB的通透性,脑微血管内皮细胞上的α-ATP合成酶是其可能的作用位点。

英文摘要：

Objective To gain an insight into the working mechanisms for endothelial monocyte-activating polypeptide-II（EMAP-II）-induced opening of blood-tumor barrier（BTB）.Methods Tumor-bearing rats were randomly divided into 4 groups（n = 12,each）：control group,EMAP-II group,oligomycin group and oligomycin ＋ EMAP-II group.Evans blue（EB） method was used to evaluate the changes in BTB permeability.The protein expression level of tight junction（TJ）-related protein ZO-1 on brain microvascular endothelial cells（BMECs） was measured by western blot assay.Double immunofluorescence was used to identify the expression and distribution of EMAP-II and α-ATP synthase on BMECs.Results Compared with control and oligomycin groups,the BTB permeability of EMAP-II group was increased significantly（0.01）,and the expression level of TJ-related proteins ZO-1 was significantly decreased（0.01）.The effects of EMAP-II above-mentioned were significantly inhibited by pretreatment with oligomycin（0.05）.Also,our data showed that both EMAP-II and α-ATP synthase were expressed on BMECs.Moreover,EMAP-II was co-localized with α-ATP synthase on the surface of BMECs.Conclusions EMAP-II may increase the BTB permeability by opening TJ,and α-ATP synthase on the BMECs surface might serve as its functional target.