东篱科研大数据发现系统（DRDS）

位置：成果数据库 > 期刊 > 期刊详情页

Preparation and Antioxidant Activity of Albumin Binding Salen Schiff-base Metal Complexes

ISSN号：1001-6538
期刊名称：Chinese Science Bulletin
时间：2013
页码：2956-2963
分类：O641.4[理学—物理化学;理学—化学] Q512.1[生物学—生物化学]
作者机构：[1]Key Laboratory of Eco-Environment-Related Polymer Materials of Ministry of Education, Key Laboratory of Polymer Materials of Gansu Province, Institute of Polymer, Northwest Normal University, Lanzhou 730070, China, [2]Lanzhou Institute of Biological Products, Lanzhou 730046, China
相关基金：This work was supported by the National Natural Science Foundation of China （21263024, 21244003）, the Program for Changjiang Scholars and Innovative Research Team in University （IRT1177）, the Gansu Science ＆ Technology Support Project （1011GKCAO17） and the Fundamental Research Funds for Universities of Gansu Province （2010-176）.
相关项目：羽毛角蛋白基功能高分子的制备与构效关系

关键词：牛血清白蛋白, 抗氧化活性, 金属配合物, 制备, 希夫碱, 紫外可见光谱, BSA, 清除活性, bovine serum albumin, Schiff-base metal complexes, biopolymer metal complexes, hydroxyl radical scavenging activity

中文摘要：

一种新奇 biopolymer 抗氧化剂(BSA/HOSalenM， M=Co， Mn， Zn ) 与变化形式被准备，它增加牛的浆液白朊(BSA ) 的抗氧化剂活动。变化形式被红外系列描绘了，紫外力的系列，荧光系列，圆形的二色性(CD ) 系列并且本国页。BSA 被用作 biopolymer 脚手架，和不可溶解的 Salen Schiff 底金属建筑群 HOSalenM 与 BSA 的氨基酸残余做轴的协作。当 HOSalenCo 的有约束力的率是不到 10 时， BSA 的结构是未改变的。HOSalenCo 变化形式给一个优秀氢氧根激进分子看(?????????? 愭業潮祰慲潺 ?? 汹 ? 漀?? 汹吗？

英文摘要：

A kind of novel biopolymer antioxidant （BSA/HOSalenM, M=Co, Mn, Zn） is prepared with conjugation, which increases the antioxidant activity of the bovine serum albumin （BSA）. The conjugations have been characterized by IR spectra, UV-Vis spectra, Fluorescence spectra, Circular dichroism （CD） spectra and Native-PAGE. The BSA is used as a biopolymer scaffold, and the insoluble Salen Schiff-base metal complexes HOSalenM make axial coordination with the amino acid residues of the BSA. The structure of the BSA is unchanged when the binding rate of HOSalenCo is less than 10. The HOSalenCo conjugations show an excellent hydroxyl radical （. OH） scavenging activity, and the activity （EC50） of BSA/HOSalenCo（10）（BSA： HOSalenCo=1 ： 10） is improved by two orders of magnitude compared with the BSA, while the activity of the BSA/HOSalenMn is weak and the BSA/HOSalenZn shows no scavenging activity.

同期刊论文项目