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中文摘要：
利用已构建陆地棉7235纤维cDNA文库测序，结合5′RACE技术从陆地棉（Gossypium hirsutum L）7235纤维中获得了1个1286bp的cDNA。序列分析结果表明，该cDNA包含一个编码368个氨基酸的开放读码框，5′非翻译区和一个带有poly（A）的3′非翻译区区域。与已报道的其它植物的脂肪酶基因具有较高的氨基酸序列同源性，命名为GhLipase，提交到GenBank，登录号为EU273298。RT-PCR分析表明，GhLipase在棉花的胚珠及纤维细胞中优势表达。Southem blot分析结果表明。GhLipase在陆地棉基因组可能存在两个拷贝。将该基因定位在四倍体棉花遗传图谱的A13染色体上。
英文摘要：
A cDNA library was constructed using fibers of Gossypium hirsutum 7235 line. The library was randomly sequenced and an expressed sequence tag （EST） with the function of lipase was obtained. The full-length cDNA encoding lipase in 7235 line was cloned by the rapid amplification of eDNA ends （RACE） technique and named GhLipase. Sequence analysis showed that the full-length cDNA was 1286 bp which contained a major open reading frame of 368 amino acids, 5＇ un-translated regions, 3＇ un-trans-lated regions, and a Poly （A） tail. The sequence was submitted to GenBank, and accession No.EU273298. It had high homology to reported lipase genes from other plants. RT-PCR analysis confirmed that the GhLipase gene was up-regulated in ovules and fibers compared with roots, stems, and leaves of 7235. Southern blot analysis showed that there were two copies of the GhLipase gene in the genome of G. hirsutum. GhLipase gene was mapped on chromosome A13 in [（TM-1×Hai7124） TM-1] population.
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