中文摘要：

为了研究酿酒酵母对外源叶绿体的摄取情况，可以通过PEG诱导酿酒酵母细胞原生质体摄取菠菜细胞离体叶绿体，然后利用光学显微镜和荧光显微镜观察融合后叶绿体、酵母，部分细胞可能存在融合情况，同时融合后细胞存在一定形态改变。通过提取菠菜细胞中的叶绿体，诱导酿酒酵母原生质体细胞对叶绿体的摄取。在荧光显微镜的观察下，由于叶绿体在有蓝光激发时可以自发红色荧光，同时酵母细胞在丫啶橙的染色条件下，在蓝光激发时显绿色，可以通过颜色初步判断细胞是否融合。

英文摘要：

In order to study the sistution that Saccharomyces cerevisiae uptake the external sources of chloroplast, through PEG-induced cell protoplasts of Saccharomyces cerevisiae cells in vitro uptake of spinach chloroplasts,and then use optical microscopy and fluorescence microscopy to observe the fusion of chloroplast and yeast, there may be some cells fusioning. At the same time the shape of certain fusion cells may change. By extracting the cells in spinach chloroplasts,induced protoplast Saccharomyces cerevisiae cells on the uptaked chloroplast. In the fluorescence microscope observation,since the chloroplast in the Blu-ray excitation can spontaneous emits red fluorescence,while yeast cells in Centre acridine orange staining conditions and in the Blu-ray emit significant green color,from which can we judge whether the cells have fusioned.