中文摘要：

目的：探讨RNA干扰技术对人卵巢癌细胞SKOV3中mTOR的表达及对细胞增殖与凋亡的影响。方法：培养SKOV3细胞系,设计合成mTOR siRNA实验分4组：正常培养组：未转染的正常培养SKOV3细胞;空白对照组：转染空脂质体的SKOV3细胞;转染组：转染mTOR siRNA的SKOV3细胞;无义对照组：转染无义siRNA的SKOV3细胞。采用Western blot法检测各组细胞中的mTOR蛋白表达水平;应用MTT法检测细胞增殖;流式细胞仪检测细胞凋亡。结果：mTOR siRNA转染组SK-OV3细胞mTOR蛋白的表达显著低于各对照组（P〈0.05）;mTOR siRNA转染组SKOV3细胞增殖低于各对照组（P〈0.05）;mTOR siRNA转染组细胞凋亡率高于各对照组（P〈0.05）。结论：mTOR siRNA对SKOV3细胞中mTOR蛋白的表达有明显的抑制作用,特异性阻断mTOR基因表达可显著抑制SKOV3细胞的增殖并促进其凋亡。

英文摘要：

Objective：To explore the effect of RNA interference technique on mTOR expression,proliferation and apoptosis of human ovarian cancer SKOV3 cells.Methods：SKOV3 cell line was cultured and divided into 4 group： normal culture group（normal SKOV2 cells without transfection）,blank control group（SKOV3 cells transfected by blank liposome）,transfection group（SKOV3 cells transfected by mTOR siRNA）,and non-sense control group（SKOV3 cells transfected by non-sense siRNA）.Western blot was used to detect the expression levels of mTOR protein in the four groups;MTT method was used to detect cell proliferation;flow cytometry was used to detect cell apoptosis.Results：The expression level of mTOR protein in transfection group was statistically significantly lower than those in the other three groups（P0.05）;the rate of cell proliferation in transfection group was statistically significantly lower than those in the other three groups（P0.05）;the rate of cell apoptosis in transfection group was statistically significantly higher than those in the other three groups（P0.05）.Conclusion：mTOR siRNA inhibits mTOR protein expression in SKOV3 cells obviously,blocking mTOR protein specifically can inhibit proliferation of SKOV3 cells and promote its apoptosis.