采用多种体外评价体系研究了补血草不同部位多酚提取物的抗氧化能力,结果表明:在4种不同的检测体系中,补血草根、根茎、叶和花的多酚提取物表现出不同的抗氧化能力水平.还原力从大至小依次为根〉根茎〉叶〉花;FRAP值和TEAC值大小顺序依次分别为根〉根茎≈叶〉花,根≈根茎≈叶〉花;而对β-胡萝卜素-亚油酸漂白的抑制作用大小为花〉叶〉根〉根茎.光谱分析显示,根、根茎和叶提取物及花提取物的主要组分分别为原花色素类和黄酮类.在不同评价体系中,补血草不同部位多酚提取物的抗氧化能力与原花色素含量(R2=0.5450—0.9201),与黄酮含量(R2=0.7764—0.9993)均呈显著正相关.可见,补血草多酚提取物具有很强的体外抗氧化能力,且活性强弱与其原花色素和黄酮含量的高低密切相美.
The antioxidant capacities of polyphenol extracts from different parts of Limonium sinense were evaluated by several in vitro systems of assay. The result showed that the polyphenol extracts from different parts had different levels of antioxidant capacity in the four systems tested. The reducing power of polyphenol extracts from different parts was in the order of root 〉 rhizome 〉 leaf 〉 flower, the ranking order of the Ferric Reducing Antioxidant Power (FRAP) value and the Trolox Equivalent Antioxidant Capacity (TEAC) value was root 〉 rhizome ≈ leaf 〉 flower, root ≈ rhizome leaf 〉 flower, respectively, while the β- earotene-linoleate bleaching inhibition was flower 〉 leaf 〉 root 〉 rhizome. Spectrum displayed that the main constituents in polyphenol extracts from root, rhizome and leaf, and flower was proanthocyanin and flavonoid, respectively. Linear correlation between antioxidant capacities of various parts in different assays and proanthocyanin content (R2 = 0. 545 0 - 0. 920 1 ) , and total flavonoid (R2 = 0. 776 4 -0. 999 3 ) was observed. Therefore, it could be concluded that polyphenol extracts from different parts of L. sinense have strong antioxidant activity in vitro, in which, high content of proanthoeyanin and total flavonoid in polyphenol extracts closely correlates with the antioxidant activity in different parts.